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以山东省41份小麦种质资源为材料,使用46对引物,通过SSR技术对其进行了DNA指纹数据库的构建。结果显示,所采用的46对引物在本试验材料中共检测到69个等位基因,有较好的多态性。利用NTSYS进行UPGMA聚类分析后,发现这41种材料在遗传相似系数0.68为阀值处可以分为3个类群。试验证明该方法能够分辨各个种质间的亲缘关系,能够较好满足小麦DNA指纹数据库的构建要求,对山东省小麦遗传资源的收集、保存、分类、评价、核心种质的建立等研究工作提供理论依据。
Using 41 wheat germplasm resources in Shandong Province as materials, 46 pairs of primers were used to construct DNA fingerprinting database by SSR technique. The results showed that 46 pairs of primers were used in this test to detect a total of 69 alleles, with good polymorphism. After UPGMA cluster analysis using NTSYS, we found that these 41 materials could be divided into three groups at the genetic similarity coefficient of 0.68. The experiment proves that this method can distinguish the genetic relationship among germplasms, and can meet the requirement of wheat DNA fingerprinting database construction. It provides the research work of collection, conservation, classification, evaluation and establishment of core collection of wheat germplasm in Shandong Province Theoretical basis.