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以重唇石斛(Dendrobium hercoglossum Rchb.f.)成熟种子为外植体,MS为基本培养基,研究了植物生长调节素种类及浓度对重唇石斛继代增殖和生根的影响。结果表明:重唇石斛最佳的启动培养基为MS+6-BA 1.5mg·L~(-1)+NAA 0.5mg·L~(-1)。利于继代增殖的培养基为MS+6-BA 2.0mg·L~(-1)+NAA 0.4mg·L~(-1)+KT 0.2mg·L~(-1)+AC 1.0g·L~(-1),最佳的生根壮苗培养基为MS+6-BA 0.5mg·L~(-1)+NAA 2.0mg·L~(-1)+AC 1.0g·L~(-1),平均根长3.12cm,生根率达到90%以上。
The mature seeds of Dendrobium hercoglossum Rchb.f. were used as explants and MS as the basic medium to study the effects of plant growth regulators and their concentrations on the proliferation and rooting of D. lugens. The results showed that the optimum medium for the initiation of Dendrobium nobile was MS + 6-BA 1.5 mg · L -1 + NAA 0.5 mg · L -1. The medium for the subsequent proliferation was MS + 6-BA 2.0 mg · L -1 + NAA 0.4 mg · L -1 KT 0.2 mg · L -1 + AC 1.0 g · L ~ (-1). The best medium for rooting and seedling growth was MS + 6-BA 0.5 mg · L -1 + NAA 2.0 mg · L -1 + AC 1.0 g · L -1 ), The average root length of 3.12cm, rooting rate of 90% or more.