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目的比较男性与女性肝细胞癌(HCC)在基因组DNA拷贝数变异(CNA)方面的差异性。方法采用高分辨率微阵列比较基因组杂交技术(array-CGH)检测17例女性与46例男性HCC患者的CNA差异。结果女性HCC染色体片段1q21.3-q22扩增频率(76.5%vs 37.0%,P=0.009)、11q11扩增频率(35.3%vs 0.0%,P=0.000 2)、19q13.31-q13.32扩增频率(23.5%vs 0.0%,P=0.004)和16p11.2丢失频率(35.3%vs 6.5%,P=0.009)显著高于男性,而男性则有更高频率的11q11丢失(63.0%vs 17.6%,P=0.002)。进一步统计分析结果显示,11q11扩增与19q13.31-q13.32扩增(P=0.042)及16p11.2丢失(P=0.033)显著相关,而1q21.3-q22扩增与19q13.31-q13.32扩增(P=0.046)显著相关。结论 CNA在性别特异性HCC演进过程中可能发挥重要作用。
Objective To compare the differences in genomic DNA copy number variation (CNA) between male and female hepatocellular carcinoma (HCC). Methods High-resolution microarray-based comparative genomic hybridization (array-CGH) was used to detect CNA differences between 17 women and 46 HCC patients. Results The frequencies of 1q21.3-q22 (76.5% vs 37.0%, P = 0.009), 11q11 (35.3% vs 0.0%, P = 0.000 2) and 19q13.31-q13.32 The frequencies of increased frequency (23.5% vs 0.0%, P = 0.004) and 16p11.2 loss frequency (35.3% vs 6.5%, P = 0.009) were significantly higher in males compared with men with higher frequency of 11q11 loss (63.0% vs 17.6 %, P = 0.002). Further statistical analysis showed that 11q11 amplification was significantly associated with 19q13.31-q13.32 amplification (P = 0.042) and 16p11.2 loss (P = 0.033), whereas 1q21.3-q22 amplification was significantly associated with 19q13.31- q13.32 amplification (P = 0.046) was significantly correlated. Conclusion CNA may play an important role in the evolution of sex-specific HCC.