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目的了解肿瘤组织在血卟啉衍生物及抗坏血酸溶液中浸泡一定时间后，血卟啉衍生物渗入瘤组织的深度、在瘤组织中的含量及抗坏血酸对其渗入和光敏作用的影响。方法以小鼠Ｓ１８０实体瘤和光敏剂癌光啉（ＰｓＤ－００７）为材料，将肿瘤组织分别浸泡在ＰｓＤ－００７（浸Ⅰ组）及ＰｓＤ－００７－抗坏血酸（浸Ⅱ组）溶液中１小时，并以静脉注入ＰｓＤ－００７２４小时的肿瘤为对照（静注组）。通过紫外光照射，观察肿瘤组织中ＰｓＤ－００７红色荧光，测定肿瘤组织中ＰｓＤ－００７和丙二醛含量。结果（１）肿瘤横剖面及肿瘤组织冰冻切片在紫外光照射下所显示的ＰｓＤ－００７红色荧光，浸Ⅰ组和浸Ⅱ组均强，且外周强于中心部位；而静注组则弱，但分布均匀。（２）肿瘤中心部组织匀浆中，ＰｓＤ－００７和丙二醛含量均为浸Ⅱ组＞浸Ⅰ组＞静注组（Ｐ＜０．０５）。结论ＰｓＤ－００７及抗坏血酸均能通过浸泡渗入肿瘤组织，抗坏血酸有促进ＰｓＤ－００７渗入肿瘤组织和增强ＰｓＤ－００７光敏反应的作用。
Objective To investigate the depth of infiltration of hematoporphyrin derivatives into tumor tissue, the content of hematoporphyrin derivatives in tumor tissue, and the effect of ascorbic acid on their infiltration and photosensitization after immersing tumor tissue in hematoporphyrin derivatives and ascorbic acid solution for a certain period of time. Methods Tumor tissues were immersed in PsD-007 (I immersion group) and PsD-007-ascorbic acid (Immersion II group) solution for 1 hour with mouse S180 solid tumor and photosensitizer photosensitizer (PsD-007) as materials. , and intravenous injection of PsD-00724 hours of tumor as a control (intravenous group). The red fluorescence of PsD-007 in the tumor tissue was observed by ultraviolet light, and the content of PsD-007 and malondialdehyde in the tumor tissue was measured. Results (1) The PsD-007 red fluorescence showed by the cross-section of tumors and frozen sections of tumor tissue under UV light irradiation was strong in both the leaching I group and the immersion II group, and the peripheral was stronger than the center; while the intravenous injection group was weak. But the distribution is even. (2) The content of PsD-007 and malondialdehyde in the homogenates of the tumor center were both in the immersion group II> leaching I group> intravenous injection group (P<0.05). Conclusion Both PsD-007 and ascorbic acid can penetrate into tumor tissue through soaking. Ascorbic acid can promote the penetration of PsD-007 into tumor tissue and enhance the photosensitizing reaction of PsD-007.