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目的探讨不同孕期大鼠血铅水平与胎盘一氧化氮(NO)及一氧化氮合酶(NOS)与胎盘绒毛超微结构的关系。方法用硝酸还原酶法、NOS测定试剂盒测定46例(分为A、B、C组)不同孕期铅暴露和17例对照组孕鼠胎盘匀浆NO、NOS水平,并对受试胎盘组织切片进行电镜检查。结果A、B组NO及NOS高于正常对照组(P均<0.01);C组NO及NOS与正常对照组比较差异无显著性(P>0.05)。阶段铅暴露组,电镜显示胎盘结构代偿增生或轻度损伤表现。全程铅暴露组血铅水平最高,胎盘结构呈现失代偿改变。结论孕期铅暴露血铅水平、胎盘局部NO/NOS水平变化与胎盘发生病理改变间关系密切,可能在围生期铅毒性发生发展中起重要作用。
Objective To investigate the relationship between serum lead levels and nitric oxide (NO), nitric oxide synthase (NOS) and placental ultrastructure in different pregnant rats. Methods Nitric acid reductase and NOS assay kit were used to determine the levels of NO and NOS in placenta of 46 pregnant women (divided into groups A, B and C) during the second trimester exposure to lead and 17 control groups. The placenta tissue sections Electron microscopy. Results NO and NOS in group A and group B were significantly higher than those in control group (all P <0.01). There was no significant difference in NO and NOS between group C and control group (P> 0.05). Stage lead exposure group, electron microscopy showed placental structure compensatory proliferation or mild injury performance. Lead exposure in the whole group had the highest level of blood lead, placental structure showed decompensation changes. Conclusion There is a close relationship between blood lead level and NO / NOS level in placenta during pregnancy and the pathological changes of placenta. It may play an important role in the occurrence and development of perinatal lead toxicity.