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目的研究转化生长因子β诱导基因(transforming growth factor-βinduced gene,TGFBI)是否能抑制人乳腺癌细胞株(MDA-MB-231)的体内外增生。方法将外源性TGFBI稳定转染到人乳腺癌细胞株(MDA-MB-231)中,测定细胞增殖率、细胞周期、软琼脂克隆形成率及P21、P53蛋白表达变化,检测乳腺癌细胞的致瘤性。结果外源性TGFBI在人乳腺癌细胞株(MDA-MB-231)中能够稳定地高表达;外源性TGFBI可以明显地抑制乳腺癌细胞因血清生长因子刺激的增生;与转染空质粒的对照组细胞V23101比较,外源性TGFBI相对软琼脂克隆形成数减少了90.89%;TGFBI可以使人乳腺癌细胞阻滞在G1期,延缓其进入S期的时间。外源性TGFBI可以延长裸鼠肿瘤发生的潜伏期,并降低肿瘤发生率。结论 TGFBI能够抑制人乳腺癌细胞株(MDA-MB-231)的体内外增生。
Objective To investigate whether transforming growth factor-βinduced gene (TGFBI) can inhibit the proliferation of human breast cancer cell line (MDA-MB-231) in vitro and in vivo. Methods Exogenous TGFBI was stably transfected into human breast cancer cell lines (MDA-MB-231). The proliferation rate, cell cycle, the formation rate of soft agar and the expression of P21 and P53 were detected. Tumorigenicity. Results Exogenous TGFBI was stably overexpressed in human breast cancer cell lines (MDA-MB-231). Exogenous TGFBI could significantly inhibit the proliferation of breast cancer cells stimulated by serum growth factors Compared with V23101 cells in control group, the number of exogenous TGFBI reduced by 90.89% compared with that of soft agar. TGFBI could arrest human breast cancer cells in G1 phase and delay their entry into S phase. Exogenous TGFBI can prolong the incubation period of tumor in nude mice and reduce the incidence of tumors. Conclusion TGFBI can inhibit the proliferation of human breast cancer cell line (MDA-MB-231) in vitro and in vivo.