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将大鼠分为空白组、模型组、反左金丸组后,模型组及反左金丸组以冰水灌胃3周,之后予冰水灌胃联合凉水泡浴2周,诱导大鼠胃寒模型形成,反左金丸组每日予反左金丸汤剂灌胃,持续5周。第6周,每组抽取12只大鼠麻醉处死后取胃,部分胃体固定后石蜡包埋并切片,进行组织学检查,部分胃组织进行酶联免疫吸附实验,观察IL-2的浓度。剩余存活大鼠用于观察模型持续时间。造模后,大鼠出现明显胃炎病变,大鼠体征和药物反证结果均符合胃寒模型指标,病理检测等结果证明以冰水灌胃结合凉水泡浴的方法可建立大鼠胃寒模型。
After the rats were divided into blank group, model group and anti-Zuojin pill group, the model group and anti-Zuojin pill group were given ice-water for 3 weeks, then the rats were treated with ice-water combined with cold water for 2 weeks, Model formation, anti-Zuo Jinwan group anti-Zuo Jintang decoction daily, for 5 weeks. At the 6th week, 12 rats in each group were killed after anesthesia, the stomach was taken, part of the gastric body was fixed and then paraffin embedded and sectioned for histological examination. Some stomach tissues were tested for enzyme-linked immunosorbent assay to observe the concentration of IL-2. Surviving remaining rats were used to observe model duration. After modeling, the rats showed obvious gastritis, the signs of the rats and the anti-evidence of the drugs were in line with the indicators of the Wei Han model, pathological examination results show that the method of ice-water combined with cold water bath rat gastric cold model can be established.