论文部分内容阅读
目的研究白花檵木醇提物(ethanol extract of Loropetalum chinensis,EELC)治疗糖尿病皮肤溃疡的分子机制。方法采用体外表皮干细胞(epidermal stem cells,ESCs)培养技术与双荧光素酶报告基因检测系统研究EELC治疗糖尿病皮肤溃疡的分子机制。结果 EELC中含有山柰酚、檞皮素、杨梅素、没食子酸。ESCs经EELC处理48 h后,Western blotting检测表明Wnt信号通路相关的Wnt、β-catenin与C-myc蛋白表达上调,且与EELC剂量呈正相关(P<0.01)。经双荧光素酶报告基因检测系统进一步验证了EELC对Wnt信号通路的靶向作用。结论 EELC激活经典Wnt信号通路,促使β-catenin关键蛋白积累,进入细胞核内与转录因子结合,激活下游特异靶基因表达,促进ESCs增殖、分化,有效地修复糖尿病溃疡皮肤创面。
Objective To study the molecular mechanism of diabetic extract of Loropetalum chinensis (EELC) on diabetic skin ulcers. Methods The molecular mechanism of EELC in the treatment of diabetic skin ulcer was studied using in vitro cultured epidermal stem cells (ESCs) and dual luciferase reporter assay. Results EELC contains kaempferol, dermatan, myricetin, gallic acid. After treated with EELC for 48 h, the expression of Wnt, β-catenin and C-myc in Wnt signaling pathway was up-regulated by Western blotting and positively correlated with the dose of EELC (P <0.01). The dual luciferase reporter gene detection system further validates the targeting effect of EELC on Wnt signaling pathway. Conclusion EELC activates the canonical Wnt signaling pathway, which promotes the accumulation of β-catenin key proteins, enters the nucleus and binds to transcription factors, activates the expression of specific downstream target genes, promotes the proliferation and differentiation of ESCs, and effectively repairs the skin wounds of diabetic ulcers.