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目的:分析大肠埃希菌(ECO)及肺炎克雷伯菌(KPN)耐药性变化趋势,以及产超广谱β-内酰胺酶(ES-BLs)和非产ESBLs菌株的药敏差异,为指导临床用药提供依据。方法:用美国临床实验室标准化委员会(NCCLS)推荐的初筛试验和表型确证试验检测ESBLs产生株,使用最低抑菌浓度法和K-B法检测临床分离的大肠埃希菌和肺炎克雷伯菌1178株对10种抗菌药物的耐药性情况。结果:我院近5年来,大肠埃希菌和肺炎克雷伯菌中ESBLs检出率分别为27.8%和16.15%,总检出率23.9%,产ESBLs菌株对10种抗菌药物的耐药率比不产酶菌株均有不同程度的增加,但所有菌株对亚胺培南都敏感。结论:产ESBLs菌株在大肠埃希菌和肺炎克雷伯菌中的检出率呈逐年上升趋势,产ESBLs菌株对所测试的10种抗菌药物的耐药程度比不产酶株为高,且呈多重耐药性,但所有菌株对亚胺培南均敏感。
OBJECTIVE: To analyze the trend of drug resistance of Escherichia coli (ECO) and Klebsiella pneumonia (KPN) and the difference of drug susceptibility between ESBLs producing strains and non-ESBLs producing strains, To guide the clinical medication to provide the basis. Methods: ESBLs-producing strains were tested by the screening test and phenotypic confirmatory test recommended by the National Committee for Clinical Laboratory Standards (NCCLS). The minimal inhibitory concentration method and KB method were used to detect the clinical isolates of Escherichia coli and Klebsiella pneumoniae 1178 strains of 10 kinds of antimicrobial resistance situation. Results: The detection rates of ESBLs in Escherichia coli and Klebsiella pneumonia in the past five years in our hospital were 27.8% and 16.15% respectively, with a total detection rate of 23.9%. The rates of ESBLs-producing strains of ESBLs against 10 antibiotics Compared with non-enzyme-producing strains were increased to varying degrees, but all strains were sensitive to imipenem. CONCLUSION: The detection rate of ESBLs-producing strains in Escherichia coli and Klebsiella pneumoniae is increasing year by year. ESBLs-producing strains are more resistant to 10 antibiotics tested than those without producing ESBLs Multi-drug resistant, but all strains were sensitive to imipenem.