论文部分内容阅读
为研究从真菌松萝中提取的松萝酸对体外培养前列腺癌PC-3M细胞增殖的抑制效应,用细胞培养、细胞生长抑制实验(MTT法)观察PC-3M细胞形态、细胞生长密度和检测细胞生长抑制率,用3H-TdR掺入法测定细胞增殖过程中DNA的含量。结果表明,与对照组相比,实验组PC-3M细胞形态异常、细胞生长密度降低,细胞生长抑制率增强,3H-TdR掺入量明显减少,并呈剂量依赖关系,与松萝酸浓度的相关系数分别为0·9799与-0·9525。结论:松萝酸对体外培养PC-3M细胞具有抑制效应,使肿瘤细胞增殖减慢,具有明显的抗肿瘤作用。
To study the inhibitory effect of usnic acid extracted from fungus Pinus sylvestris on the proliferation of PC-3M cells cultured in vitro, the morphology and cell density of PC-3M cells were observed by cell culture and cell growth inhibition assay (MTT) Cell growth inhibition rate, using 3H-TdR incorporation assay DNA content in cell proliferation. The results showed that compared with the control group, PC-3M cells in the experimental group were abnormal in morphology, cell growth density was reduced, cell growth inhibition rate was increased, 3H-TdR incorporation was significantly reduced, and in a dose-dependent manner, with the concentration of usnic acid The correlation coefficients were 0.9799 and -0.9525, respectively. Conclusion: Usnic acid has inhibitory effect on PC-3M cells cultured in vitro, which makes the proliferation of tumor cells slow down and has obvious anti-tumor effect.