本文采用细胞计数和中性红摄取法观察了ＢＰ７，８ｄｉｏｌ对ＸＥＭ２（稳定表达了大鼠肝细胞色素４５０１Ａ１）和Ｖ７９（未表达Ｐ４５０１Ａ１）细胞生长的作用，目的为建立体外细胞毒模型。结果表明，在１．０、２．５、５．０μｍｏｌ·Ｌ１不同浓度下，ＢＰ７，８ｄｉｏｌ对ＸＥＭ２细胞产生很强的毒性，其毒性具有浓度和时间依赖性，在相同的浓度下ＢＰ７，８ｄｉｏｌ对Ｖ７９细胞并没有影响，同时也发现，细胞色素Ｐ４５０酶抑制剂αＮＦ对ＢＰ７，８ｄｉｏｌ引起的细胞毒具有保护作用。这些结果说明，ＢＰ７，８ｄｉｏｌ对ＸＥＭ２细胞毒性是通过细胞色素Ｐ４５０１Ａ１的作用引起的。 In this paper, the use of cell counting and neutral red uptake observed BP  7,8  dioxan XEM2 (stable expression of rat liver cytochrome 4501A1) and V79 (not expressed P4501A1) cell growth in order to establish in vitro cytotoxic model. The results showed that under different concentrations of 1.0,2.5,5.0μmol·L1, BP7,8diol had strong toxicity on XEM2 cells in a concentration-dependent and time-dependent manner. In the same The concentration of BP  7,8  diarrhea V79 cells did not affect, but also found that cytochrome P450 enzyme inhibitors  α  NF 7  8diol induced cytotoxicity has a protective effect. These results suggest that the cytotoxicity of BP-7,8-diol to XEM2 cells is caused by the action of cytochrome P4501A1.