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目的：研究游离脂肪酸（油酸和软脂酸）对基础状态大鼠β细胞胰岛素分泌和前胰岛素原ｍＲＮＡ表达的影响。方法：体外分离培养Ｗｉｓｔａｒ乳鼠β细胞，与软脂酸或油酸共同孵育２４ｈ后，测定胰岛素浓度和３ＨＴｄＲ掺入量，Ｎｏｒｔｈｅｒｎ杂交检测前胰岛素原ｍＲＮＡ表达。结果：（１）软脂酸（０．２５ｍｍｏｌ／Ｌ）或油酸（０．１２５ｍｍｏｌ／Ｌ）组β细胞胰岛素分泌量明显高于对照组。（２）经软脂酸和油酸共同培养２４ｈ后，β细胞３ＨＴｄＲ掺入量增高。（３）软脂酸和油酸试验组β细胞前胰岛素原ｍＲＮＡ表达均高于未经游离脂肪酸培养的对照组。结论：游离脂肪酸可能通过促进基础状态β细胞前胰岛素原ｍＲＮＡ表达和细胞增生，造成基础状态β细胞分泌胰岛素过多，从而参与了高胰岛素血症的发生。
Objective: To study the effect of free fatty acids (oleic acid and palmitate) on the β-cell insulin secretion and procollagen mRNA expression in basal rats. Methods: The β cell of Wistar suckling rat was isolated and cultured in vitro. After co-incubated with palmitate or oleic acid for 24 hours, the insulin concentration and 3H-TdR incorporation were measured. The mRNA expression of proinsulin before Northern hybridization was detected. Results: (1) The insulin secretion of β cells in palmitic acid (0.25 mmol / L) or oleic acid (0.125 mmol / L) group was significantly higher than that in control group. (2) 3H-TdR incorporation of β cells increased after co-cultured with palmitate and oleic acid for 24h. (3) The pre-proinsulin mRNA expression in the palmitate and oleic acid groups was higher than that in the control group without free fatty acid. Conclusion: Free fatty acids may participate in the pathogenesis of hyperinsulinemia by promoting the expression of pro-proinsulin mRNA and cell proliferation of basal β-cells, resulting in excessive secretion of insulin in basal β cells.