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目的 观察缺氧性肺动脉高压大鼠泡内肺动脉与右心室胶原表达及黄芪的干预作用 ,初步探讨其作用机制。方法 将雄性Wistar大鼠随机分为 3组 :( 1)缺氧组 :在常压缺氧舱内缺氧 ,每日 12小时 ,连续 3周 ;( 2 )黄芪组 :缺氧条件同缺氧组 ,自缺氧第一天起给大鼠每天腹腔注射 0 2ml黄芪注射液 ;( 3 )正常对照组 :室内空气正常饲养。用心导管和Fick’s法测定肺动脉压和右心排血量 ,苦味酸天狼猩红染色切片观察泡内肺动脉胶原表达 ,逆转录聚合酶链反应 (RT PCR)检测右心室胶原mRNA、肺组织内皮素 1(ET 1)和一氧化氮合酶 (NOS)mRNA的表达。结果 ( 1)与正常对照组比较 ,缺氧组肺动脉压明显升高 [( 2 4 8± 3 6)mmHg,( 18 8± 1 7)mmHgP <0 0 1],泡内肺动脉胶原表达明显增多 [( 2 0 4± 4 5 )AU ,( 3 4± 1 1)AUP <0 0 1],右心室I型胶原mRNA表达增高 ,肺组织中ET 1mRNA表达增加 ,而NOSmRNA表达下降 ;( 2 )与缺氧组比较 ,黄芪组右心排血量明显升高 (分别为 14 1± 2 0 )ml/min ,( 118± 4 0 )ml/min (P <0 0 1) ,泡内肺动脉胶原表达明显减少 (分别为 5 6± 1 2 )AU ,( 2 0 4± 4 5 )AU (P <0 0 0 1) ,右心室I型胶原的mRNA表达明显下降 ,肺组织中ET 1mRNA表达明显下降 ,而NOS的mRNA表达升高。结论 ?
Objective To observe the expression of collagen in pulmonary arteries and right ventricle of rats with hypoxic pulmonary hypertension and the intervention of Astragalus membranaceus, and to explore its possible mechanism. Methods Male Wistar rats were randomly divided into three groups: (1) hypoxia group: hypoxia in normobaric hypoxia chamber for 12 hours a day for 3 weeks; (2) Astragalus group: Group, from the first day after hypoxia to rats daily intraperitoneal injection of 0.2ml astragalus injection; (3) normal control group: normal indoor air feeding. Pulmonary arterial pressure and right cardiac output were measured by cardiac catheterization and Fick’s method. Collagen mRNA was detected by picric acid Sirius Red staining, and collagen mRNA expression in right ventricle and endothelin-1 in lung tissue were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) (ET 1) and nitric oxide synthase (NOS) mRNA expression. Results (1) Compared with the normal control group, the pulmonary arterial pressure was significantly increased in hypoxia group [(24.8 ± 36) mmHg, (18.8 ± 17) mmHgP <0.01) (2 0 4 ± 4 5) AU, (3 4 ± 1 1) AUP <0 0 1]. The mRNA expression of type I collagen in the right ventricle increased and the expression of ET 1 mRNA increased while the expression of NOSmRNA decreased in the lung tissue. (2) Compared with the hypoxia group, the right cardiac output of astragalus was significantly increased (14 1 ± 20 ml / min, (118 ± 40) ml / min, P <0.01) (P <0.01). The mRNA expression of type I collagen in the right ventricle was significantly decreased, and the expression of ET 1 mRNA in the lung tissue was significantly higher than that in the control group Decreased, while NOS mRNA expression increased. in conclusion ?