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第二类肽链释放因子eRF3(eukaryotic polypeptide release factor)是一种GTPase,它促进第一类肽链释放因子eRF1的释放活性,并与细胞周期调控、细胞骨架组装、细胞凋亡和肿瘤形成等过程相关。哺乳动物细胞中eRF3有两种——eRF3a和eRF3b,分别由GSPT1和GSPT2(G1 to S phase transition 1/2)基因编码。生存素(survivin)是迄今发现的最强有力的凋亡抑制因子,具有独特的结构和复杂的功能,不仅可以抑制细胞凋亡,还参与细胞有丝分裂、血管的生成等过程。eRF3和survivin都与细胞周期和细胞凋亡的调控相关。该实验室的前期研究表明,eRF3和survivin具有相互作用关系。该研究进一步对eRF3a进行截短突变,采用酵母双杂交和pull-down两种分析方法依次验证eRF3a(1-72aa)和eRF3a(1-36aa)与survivin的相互作用关系。结果表明,eRF3a(1-72aa)和eRF3a(1-36aa)均可以与survivin相互作用,由此确定eRF3a与survivin相互作用的最小结构域位于其N末端1-36aa之间,从而为进一步证实eRF3a的N端结构域与survivin协同作用参与细胞周期和细胞凋亡的调控提供了数据支持。
The second type of eukaryotic polypeptide release factor eRF3 (eukaryotic polypeptide release factor) is a GTPase that promotes the release of eRF1, a first class of peptide chain releasing factor, and is associated with cell cycle regulation, cytoskeletal assembly, apoptosis and tumorigenesis Process related. There are two eRF3s in mammalian cells, eRF3a and eRF3b, encoded by the GSPT1 and GSPT2 (G1 to S phase transition 1/2) genes, respectively. Survivin is the most powerful inhibitor of apoptosis found so far. It has a unique structure and complex functions. It not only inhibits apoptosis, but also participates in cell mitosis and angiogenesis. Both eRF3 and survivin are involved in the regulation of cell cycle and apoptosis. Previous studies in the laboratory showed that eRF3 interacts with survivin. In this study, we further truncated eRF3a and verified the interaction of eRF3a (1-72aa) and eRF3a (1-36aa) with survivin by yeast two-hybrid and pull-down assays. The results showed that both eRF3a (1-72aa) and eRF3a (1-36aa) could interact with survivin, thus confirming that the minimal domain of eRF3a interacting with survivin is located between its N-terminus and 1-36aa. To further confirm that eRF3a N-terminal domain and survivin synergistically involved in the regulation of cell cycle and apoptosis provide data support.