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目的:探讨2型糖尿病患者血小板表面CD41,CD62p表达,内皮细胞损伤标志物变化及其临床价值。方法:用流式细胞术测定患者血小板表面CD41,CD62p;用放射免疫法检测血清ET-1;用ELISA方法检测血浆t-PA,PAI-1;统计学方法:所有实验数据采用SPSS13.0统计软件处理,用均数±标准差(x±s)表示;采用单因素方差分析,两组间比较采用t检验,P<0.05有统计学意义。结果:糖尿病组血小板表面CD62P的表达、血浆PAI-1水平均较正常组增高(P<0.05),而t-PA水平较正常组降低(P<0.05);糖尿病组血小板表面CD41的表达,血清ET-1水平与正常组比较无显著性差异(P>0.05);糖尿病伴血管病变组血小板表面CD62P的表达、血浆PAI-1水平均较不伴血管病变组增高(P<0.05);糖尿病伴血管病变组序小板表面CD41的表达、血浆t-PA水平、血清ET-1水平与不伴血管病变组指标比较没有显著性差异(P>0.05)。结论:探讨血栓前状态若干相关分子标志物即血浆内皮素-1(ET-1)、血小板α-颗粒膜蛋白(GMP-140)、组织纤溶酶原激活物(t-PA)及其抑制物(PAI-1)及变化,探讨血小板活化在糖尿病患者中的临床意义。
Objective: To investigate the changes of platelet surface CD41 and CD62p expression, endothelial cell injury markers and their clinical value in type 2 diabetic patients. Methods: The platelet surface CD41 and CD62p were measured by flow cytometry. The level of ET-1 in serum was detected by radioimmunoassay. The levels of t-PA and PAI-1 in plasma were determined by ELISA. All the data were analyzed by SPSS13.0 Software processing, with mean ± standard deviation (x ± s) said; using one-way ANOVA, t-test was used to compare the two groups, P <0.05 was statistically significant. Results: The expression of CD62P on platelets and the level of plasma PAI-1 in diabetic group were significantly higher than those in normal group (P <0.05), while the level of t-PA was lower in normal group (P <0.05) There was no significant difference in the level of ET-1 between the two groups (P> 0.05). The expression of CD62P on platelet surface and the level of plasma PAI-1 in diabetic patients with vascular lesion were significantly higher than those without angiopathy (P <0.05) There was no significant difference in CD41 expression, plasma t-PA level, serum ET-1 level and angiopathy group without angiopathy in vascular lesions group (P> 0.05). Conclusion: To investigate the relationship between thrombosis state related molecular markers such as ET-1, GMP-140, t-PA, (PAI-1) and changes, to explore the clinical significance of platelet activation in diabetic patients.