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目的探讨香莲复方及其君药的主要成分丁香酚、小檗碱的抗真菌及逆转氟康唑耐药作用。方法采用香莲外洗液、丁香酚、小檗碱、丁香酚和小檗碱的混合液分别连续诱导培养白念珠菌临床分离氟康唑耐药株,采用液基微量稀释法测定氟康唑对原代及诱导菌株的最低抑菌浓度(MIC)值。观察药物对耐药白念珠菌的逆转作用,比较各组诱导药物之间的作用差异。结果耐药株L1~L3对氟康唑的MIC为64μg/m L,香莲外洗液的MIC为3.906 3~7.812 5 mg/m L,丁香酚为400~800 mg/m L,小檗碱为32~64 mg/m L,丁香酚和小檗碱混合液为200/16~400/32 mg/m L。香莲外洗液、丁香酚、小檗碱以及丁香酚和小檗碱混合液对临床分离耐药菌株可在连续诱导培养6~8代实现对氟康唑逆转耐药。耐药原代菌株与其敏感子代对于诱导药物的MIC没有明显上升趋势。结论香莲外洗液、丁香酚、小檗碱以及丁香酚和小檗碱混合液具有抗真菌作用,并可逆转耐药白念珠菌对氟康唑的敏感性,以香莲外洗液复方疗效最佳。
Objective To investigate the antifungal effects of eugenol and berberine, the major constituents of the lotus root compound and its monarch herb, and to reverse the drug resistance of fluconazole. Methods Fluconazole-resistant clinical isolates of Candida albicans were continuously induced by mixed liquor of lotus lotion, eugenol, berberine, eugenol and berberine. Fluconazole was determined by liquid micro-dilution method Minimum inhibitory concentration (MIC) value for primary and induced strains. To observe the reversal effect of drug on Candida-resistant Candida, and to compare the difference between the induced drugs in each group. Results MIC of L1-L3 to fluconazole was 64 μg / ml, MIC of lotion was 3.906 3 ~ 7.812 5 mg / ml, eugenol was 400 ~ 800 mg / ml, The base was 32-64 mg / m L, and the mixture of eugenol and berberine was 200/16 ~ 400/32 mg / m L. Xianglian external lotion, eugenol, berberine and eugenol and berberine mixture of clinical isolates of drug-resistant strains can be induced by continuous induction of 6 to 8 generations of reversal of fluconazole resistance. The MICs of the drug-resistant primary strains and their susceptible offspring did not show any significant upward trend toward drug induction. Conclusion The lotus lotion, eugenol, berberine and eugenol and berberine mixture have antifungal effect and can reverse the sensitivity of Candida albicans to fluconazole, The best effect.