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目的:研究复尔康注射液对人肝癌细胞株增殖及裸鼠移植瘤生长的影响。方法:采用CCK8试剂盒检测复尔康注射液对人肝癌细胞株SMMC-7721和BeL-7402增殖的抑制作用;建立SMMC-7721细胞裸鼠移植瘤模型,通过比较肿瘤体积(tumor volume,TV)、相对肿瘤体积(relative tumor volume,RTV)和相对肿瘤增殖率(T/C)考察复尔康注射液对肿瘤生长的影响。结果:复尔康注射液对SMMC-7721和BeL-7402的IC50分别为(61.51±1.58),(68.39±7.16)mg·L-1;不同剂量复尔康注射液能够不同程度地抑制裸鼠移植瘤的生长,与阴性对照组比较,P<0.05。低、中、高剂量复尔康注射液组(0.065,0.130,0.260 g·kg-1)T/C分别为57.75%,39.9%和44.08%。结论:复尔康注射液体外能抑制人肝癌细胞株的增殖,体内能明显抑制SMMC-7721细胞裸鼠移植瘤的生长。
Objective: To study the effects of fuerkang injection on the proliferation of human hepatocellular carcinoma cell lines and the growth of xenografts in nude mice. Methods: CCK8 kit was used to detect the inhibitory effect of fuerkang injection on the proliferation of human hepatocellular carcinoma cell lines SMMC-7721 and BeL-7402. The nude mouse xenograft model of SMMC-7721 cells was established. By comparing the tumor volume (TV) , The relative tumor volume (RTV) and the relative tumor growth rate (T / C) were used to investigate the effect of Fuerkang injection on tumor growth. Results: The IC50 of Fuerkang injection to SMMC-7721 and BeL-7402 were (61.51 ± 1.58) and (68.39 ± 7.16) mg · L-1, respectively. Different doses of fuerkang injection could inhibit the growth of nude mice The growth of xenografts was compared with the negative control group, P <0.05. The T / C of low, middle and high doses of Fuerkang injection group (0.065,0.130,0.260 g · kg-1) were 57.75%, 39.9% and 44.08%, respectively. Conclusion: Fu Er Kang injection in vitro can inhibit the proliferation of human hepatoma cell lines in vivo SMMC-7721 cells can significantly inhibit the growth of nude mice xenografts.