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为研究浙江省稻瘟病菌群体的遗传多样性,利用分布于稻瘟病菌7条染色体上的28对SSR引物,对浙江省11个城市1978至2011年11个城市的稻瘟病菌的遗传结构多样性进行了分析。结果表明,28对SSR引物共扩增出171个等位基因,每对引物可扩出2~24个等位基因,平均多样性指数0.534,平均多态性信息含量为0.494。分离于不同地理位置的稻瘟病菌株的多样性较高,其中丽水和杭州的遗传多样性最高,舟山的遗传多样性最低。分离于不同年份的稻瘟病菌的遗传结构差异较大,1990年的遗传多样最高,1978年的遗传多样性最低。分离于不同水稻类型的稻瘟病菌的遗传多样性较高,部分分离于籼稻和粳稻的稻瘟病菌的遗传结构相似甚至相同,分离于籼稻的稻瘟病菌的遗传多样性高于分离于粳稻的稻瘟病菌。
In order to study the genetic diversity of Magnaporthe grisea in Zhejiang Province, 28 SSR primers distributed on 7 chromosomes of Magnaporthe grisea were used to analyze the genetic diversity of Magnaporthe grisea from 11 cities in Zhejiang Province from 1978 to 2011 Sexuality was analyzed. The results showed that a total of 171 SSR primers amplified 171 alleles and 2 to 24 alleles per primer pair. The average diversity index was 0.534, and the average polymorphism information content was 0.494. The isolates of Magnaporthe grisea isolated from different geographical locations had higher diversity, among which Lishui and Hangzhou had the highest genetic diversity and Zhoushan had the lowest genetic diversity. The genetic structure of Magnaporthe grisea isolated in different years was quite different, with the highest genetic diversity in 1990 and the lowest genetic diversity in 1978. The genetic diversity of Magnaporthe grisea isolates from different rice types was high. The genetic structure of Magnaporthe grisea isolates partially isolated from indica and japonica were similar or even identical. The genetic diversity of Magnaporthe grisea isolated from indica rice was higher than that isolated from japonica rice Magnaporthe grisea.