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目的选择合适的实验室支原体(MP)感染的检测方法及试剂盒。方法使用日本富士MYCOⅡ和珠海丽珠试剂盒及康华试剂盒做比对,研究快速金标法与支原体抗体滴度对临床诊断支原体感染的作用。结果胶体金试剂盒之间对肺炎支原体的检出率无显著性差异,抗体滴度试剂盒在MP诊断中占优势,与快速金标法差异有统计学意义(P<0.05)。结论 MP检测方法各有利弊,实验室应结合临床要求与产品性能综合判断。
Objective To select the appropriate method and kit for detection of Mycoplasma pneumoniae (MP) infection in laboratory. Methods Using Japan Fuji MYCOⅡ and Zhuhai Livzon kit and Kanghua kit for comparison, the rapid gold standard method and mycoplasma antibody titer on the clinical diagnosis of mycoplasma infection. Results There was no significant difference in the detection rate of Mycoplasma pneumoniae between the colloidal gold kits. The antibody titer kit was predominant in the diagnosis of MP, which was significantly different from the rapid gold standard (P <0.05). Conclusion MP detection methods have their own advantages and disadvantages, laboratory should be combined with clinical requirements and product performance comprehensive judgment.