论文部分内容阅读
目的 探讨镉对肾组织中Ca2 + ATPase的影响及尼莫地平 (Nimo)对其的干预作用。方法 在体外条件下 ,测定CdCl2 对肾组织Ca2 + ATPase及乳酸脱氢酶 (LDH)、碱性磷酸酶 (ALP)、尿N 乙酰 D 氨基葡萄苷酶 (NAG)等多种肾损伤标志酶活力的作用及Nimo干预处理后的影响。结果 在低浓度CdCl2 组 ,Ca2 + ATPase的活力明显升高 ,10 0mg/LCdCl2 组Ca2 + ATPase的活力为(4 2 .12± 3.35 ) μmolPi·g-1·h-1,与对照组 [(31.2 3± 2 .5 9) μmolPi·g-1·h-1]的差异有显著性 (P <0 .0 5 ) ;而随CdCl2 浓度升高 ,Ca2 + ATPase活力受到明显抑制 ,2 0 0mg/LCdCl2 组的Ca2 + ATPase活力为 (2 0 .6 7± 2 .46 ) μmolPi·g-1·h-1,明显低于对照组 ,差异有显著性 (P <0 .0 5 )。而染镉前经Nimo预处理后 ,2 0 0mg/LCdCl2 +Nimo组的NAG活力 ,10 0、15 0、2 0 0mg/LCdCl2 +Nimo组的LDH活力及 15 0、2 0 0mg/LCdCl2 +Nimo组的ALP活力均低于单纯染CdCl2 组 ,差异有显著性 (P <0 .0 5 ) ;10 0mg/LCdCl2 +Nimo组的Ca2 + ATPase活力低于、而 2 0 0mg/LCdCl2 +Nimo组则高于单纯CdCl2 组 ,差异均有显著性 (P <0 .0 5 )。结论 CdCl2 对Ca2 + ATPase活力的影响是双相的 ,Nimo可部分解除CdCl2 对Ca2 + ATPase活力的抑制 ,并具有减轻镉?
Objective To investigate the effect of cadmium on Ca2 + -ATPase in renal tissues and the effect of Nimodipine on it. Methods In vitro, CdCl2 was used to measure the activity of Ca2 + -ATPase and renal enzymes such as lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and urine N-acetylglucosaminidase (NAG) And the impact of Nimo intervention. The results showed that the activity of Ca2 + ATPase was significantly increased in the low concentration CdCl2 group, the activity of Ca2 + ATPase in the 10 mg / L CdCl2 group was (4.12 ± 3.35) μmol Pg · h-1, compared with the control group [( 31.2 3 ± 2.59 μmolPi · g-1 · h-1] (P <0.05). With the increase of CdCl2 concentration, the activity of Ca2 + ATPase was significantly inhibited. The activity of Ca2 + ATPase in / LCdCl2 group was (20.76 ± 2.46) μmolPi · g-1 · h-1, which was significantly lower than that in the control group (P <0.05). The NAG activity in the 200 mg / L CdCl2 + Nimo group, the LDH activity in the 10 0,15 0,0 0 mg / L CdCl2 + Nimo group, and the concentration of 150,220 mg / L CdCl2 + Nimo The ALP activity of the group was lower than that of the simple CdCl2 group (P <0.05). The activity of Ca2 + ATPase of the group of 100 mg / L CdCl2 + Nimo was lower than that of the group of 200 mg / L CdCl2 + Nimo Higher than pure CdCl2 group, the difference was significant (P <0. 05). Conclusion The effect of CdCl2 on the activity of Ca2 + ATPase is biphasic. Nimo can partially relieve the inhibition of Ca2 + ATPase activity by CdCl2 and has the effect of reducing Cd2 +.