目的:扩增全长HBV DNA基因组,建立中国株HBV感染性克隆,阐述HBV基因组变异对其不同基因生物学特性影响。方法:TD-PCR和XL-PCR技术一次扩增HBV全长基因组,克隆,PCR、酶切和测序鉴定。结果:通过PCR、酶切、测序鉴定和真核细胞转染分析,获得全长HBV基因组克隆。结论:获得了HBV全基因组克隆,在真核细胞中可以表达HBsAg,为建立HBV感染性克隆奠定物质基础。 OBJECTIVE: To amplify the full-length HBV DNA genome and establish an HBV-infected clone of Chinese strain. The effects of HBV genome variation on the biological characteristics of different genes were described. Methods: The full-length HBV genome was amplified by TD-PCR and XL-PCR. Clone, PCR, digestion and sequencing were used to identify the genome of HBV. Results: Full-length HBV genome clones were obtained by PCR, restriction enzyme digestion, sequencing analysis and eukaryotic cell transfection assay. Conclusion: The whole genome of HBV was obtained and HBsAg could be expressed in eukaryotic cells, which laid the material foundation for the establishment of HBV infectious clones.