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目的 探讨尿中萘及其代谢产物作为焦炉工生物监测指标的可行性。方法 在某焦化厂随机选取 2 8名焦炉工人和 2 2名对照个体 ,统一收集工作周末班后 2h尿 ,并使用调查表收集一般情况。采用顶空固相微提取结合气相色谱 -质谱联机方法 ,同时测定尿中萘和芘的水平 ,采用酶水解结合气相色谱 -质谱联机方法 ,同时测定样品中 1-萘酚、 2 -萘酚和 1-羟基芘的水平。使用多元线性回归分析不同工种和吸烟量对尿中这 5种多环芳烃生物标志物浓度的影响。结果 尿中萘、 1-萘酚、 2 -萘酚和 1-羟基芘的浓度呈炉顶工 >炉侧和炉底工 >对照个体的趋势 ,其中尿中 1-萘酚和 1-羟基芘的水平受工种的影响大于2 -萘酚 ;不同暴露水平组中 ,吸烟个体尿中 1-萘酚、 2 -萘酚和 1-羟基芘的浓度均大于不吸烟者 ,但吸烟对 2 -萘酚的影响最大。结论 尿中 1-萘酚和 2 -萘酚浓度均能够有效地反映个体短期多环芳烃暴露的内剂量水平 ,可用于焦炉工的生物监测。
Objective To investigate the feasibility of urine naphthalene and its metabolites as biological monitoring indicators for coke oven workers. Methods A total of 28 coke oven workers and 22 control subjects were randomly selected from a coke plant. Urine was collected 2 hours after work weekend and the general conditions were collected using a questionnaire. Simultaneous determination of naphthalene and pyrene in the urine by headspace solid phase microextraction combined with gas chromatography - mass spectrometry on - line method was used to determine the contents of 1 - naphthol, 2 - naphthol and 2 - naphthol in the samples by enzymatic hydrolysis combined with gas chromatography - mass spectrometry 1-hydroxy-pyrene level. Multivariate linear regression was used to analyze the effects of different types of job and cigarette smoking on the concentrations of these five polycyclic aromatic hydrocarbons biomarkers in urine. Results The concentrations of naphthalene, 1 - naphthol, 2 - naphthol and 1 - hydroxypyrene in urine showed the tendency of top - firing, hearth and bottom> control individuals, among which 1 - naphthol and 1 - The levels of 1 - naphthol, 2 - naphthol and 1 - hydroxy - pyrene in urine were higher in non - smoker than in non - smoker at different levels of exposure, but smoking had no significant effect on 2 - naphthalene Phenol has the greatest impact. Conclusion The urinary 1 - naphthol and 2 - naphthol concentrations can all effectively reflect the internal dosages of short - term PAHs exposure and can be used for biological monitoring of coke oven workers.