目的:建立布氏田鼠实验动物化的饲育方法。方法:我们引入布氏田鼠后,采用药物净化法控制其体内外的微生物和寄生虫进行实验动物化,确定了在屏障环境中针对该动物的饲养、配种、麻醉以及输精管结扎的技术流程;并检测了布氏田鼠的主要脏器系数指标。结果:初步建立了布氏田鼠人工饲养的清洁级封闭群。群体内成年雄性布氏田鼠的肝脏、肾脏和脾脏的器官系数与雌性的差异达到显著性水平(P<0.05)。结论:本实验确定的饲育方法为同类鼠种的实验动物化工作提供了参考。 OBJECTIVE: To establish animal breeding method of Brandt’s vole Methods: After the introduction of Brandt’s vole (Microtus brandti), we used the drug purification method to control the microorganisms and parasites in vitro and in vivo, and established the technical process of feeding, breeding, anesthesia and vasectomy in the barrier environment. The main organ coefficient index of Brandt’s vole (Microtus brandti) was tested. Results: The clean-grade cloned herd of Microtus brandti was established. The differences of organ coefficient and females in liver, kidney and spleen of adult Brandt’s vole (Microtus brandti) were significant (P <0.05). Conclusion: The feeding method determined in this experiment provides a reference for laboratory animals of similar species.