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以‘资阳’香橙(Citrus junos‘Ziyang’)为试材,分别对Cit ERF9和Cit AP2-7进行了分析。结果表明:Cit ERF9基因的开放阅读框(ORF)为735 bp,含有1个外显子,可编码244个氨基酸残基的多肽。Cit AP2-7的开放阅读框为1 080 bp,具有6个外显子,可编码360个氨基酸残基。同源分析结果显示,这两个基因编码的蛋白与大豆、蓖麻、碧桃、苜蓿等植物中的同源蛋白有81%~84%的相似性。实时定量分析表明:Cit ERF9和Cit AP2-7在植株不同组织间的表达具有明显差异。在根中,Cit ERF9受各种胁迫处理诱导,而Cit AP2-7主要受ABA、Na Cl、脱水等的诱导。在叶中,各种胁迫均对Cit ERF9具有诱导作用,其模式与根中相似,而Cit AP2-7的表达则主要受ABA、ACC、Me JA、SA等激素以及低温和Na Cl的抑制。试验结果表明,Cit ERF9和Cit AP2-7参与了激素和非生物胁迫的响应过程。
Taking Citrus junos ’Ziyang’ as the test material, Cit ERF9 and Cit AP2-7 were analyzed respectively. The results showed that the open reading frame (ORF) of Cit ERF9 gene was 735 bp, containing one exon and encoding a polypeptide of 244 amino acid residues. The open reading frame of Cit AP2-7 is 1 080 bp with 6 exons encoding 360 amino acid residues. Homology analysis showed that the proteins encoded by these two genes shared 81% -84% similarities with homologous proteins in plants such as soybean, castor, bitter peach, alfalfa and so on. Quantitative real-time analysis showed that the expression of Cit ERF9 and Cit AP2-7 in different tissues of plants were significantly different. In roots, Cit ERF9 was induced by various stress treatments, whereas Cit AP2-7 was mainly induced by ABA, NaCl, dehydration and the like. In leaves, various stresses induced the induction of Cit ERF9 in a similar manner to that of roots, while expression of Cit AP2-7 was mainly inhibited by ABA, ACC, Me JA, SA and other hormones as well as hypothermia and NaCl. The results show that Cit ERF9 and Cit AP2-7 participate in the response process of hormone and abiotic stress.