论文部分内容阅读
目的 :探讨原子力显微镜在口腔变形链球菌研究中的应用。方法 :应用原子力显微镜在自然干燥状态和在态条件下观察变形链球菌血清c型MT6R菌株和MT814 8葡糖基转移酶基因缺陷型菌株B2 9的超微结构。结果 :原子力显微镜在自然干燥观察变形链球菌MT6R可见 ,表面形态呈不规则状 ,有大量的不定性物质 ,有呈囊状突起 ,菌细胞之间靠大量的不定性物质相互连在一起 ;水不溶性葡糖基转移酶 (GTFI)缺陷型菌株B2 9菌细胞表面相对光滑 ,无囊状突起 ,有颗粒状物 ,菌细胞间不定性物质较少。在液态条件下观察细菌的超微结构与自然干燥下的相似 ,但更加清晰。结论 :应用原子力显微镜可以在液态条件下观察变形链球菌表面超微结构 ,还可以对菌细胞进行三维成像
Objective: To investigate the application of AFM in the study of Streptococcus mutans. Methods: The ultrastructures of Streptococcus mutans serotype C MT6R and MT814 8-glucosyltransferase B2 9 were observed under atomic force microscope under natural drying and in the state. Results: Atomic force microscope showed that Streptococcus mutans MT6R was observed in natural dryness. The morphology of the surface was irregular. There were a large number of indefinite substances and cysts. The bacterial cells were connected together by a large amount of indefinite substances. Water The insoluble bacterial glucosyltransferase (GTFI) -deficient strain B2 9 was relatively smooth on the surface of the cells, with no cystic protrusions and granules, and less bacterial interstitial material. Bacterial ultrastructure observed under liquid conditions is similar to that under natural drying, but is clearer. Conclusion: AFM can be used to observe the surface ultrastructure of Streptococcus mutans under liquid condition, and also can carry out three-dimensional imaging of bacterial cells