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为研究猪脾转移因子(TF)对禽疫苗的免疫增强作用,采用血凝抑制(HI)试验和琼脂凝胶扩散沉淀(AGP)试验方法测试了TF分别与鸡新城疫(ND)病毒活疫苗、禽流感(AI)病毒灭活疫苗、鸡传染性支气管炎(IB)病毒灭活疫苗、鸡传染性法氏囊病(IBD)病毒活疫苗联合接种SPF鸡后抗体效价(log2x)动态变化(共4个试验组),同时运用ELISA法评估了所采用免疫程序(上述前3种疫苗与TF同时接种后第7天,进行上述第4种疫苗与TF的联合接种)对SPF鸡外周血IFN-γ、IL-2、IL-4和IL-10的影响(试验组5),于接种上述第4种疫苗后的7,14,21,28,35,42d检测细胞因子,均设立对照组(上述禽疫苗单独接种)和空白组(非疫苗接种)。结果显示,接种后7,14,21,28,35,42d,试验组的ND血凝抑制(HI)、AI HI、IB HI和IBD琼脂扩散沉淀(AGP)抗体效价均显著高于对照组(P<0.05),分别提高了1.1~1.4,1.0~1.3,0.7~1.4,0.9~1.6;试验组的IL-2、IFN-γ和IL-4浓度分别于接种后7,14,21d显著高于对照组和空白组(P<0.05),各组的IL-10浓度差异不显著。研究结果表明,TF可增强NDV活疫苗、AIV灭活疫苗、IBV灭活疫苗和IBDV活疫苗的免疫效果。
In order to study the immune enhancement effect of porcine spleen transfer factor (TF) on avian vaccines, the relationship between TF and live ND vaccine, avian influenza virus (NDV) (AI) virus inactivated vaccine, IBV inactivated vaccine and IBD virus live vaccine combined with SPF chickens, the antibody titer (log2x) 4 experimental groups). Meanwhile, the immune procedure adopted was evaluated by ELISA (the combination of the above-mentioned fourth vaccine and TF was carried out on the 7th day after the above three kinds of vaccines were simultaneously inoculated with TF) (experimental group 5). Cytokines were detected at 7, 14, 21, 28, 35, and 42 days after inoculation of the above-mentioned fourth vaccine, and control groups ( The above-mentioned bird vaccination alone) and the blank group (non-vaccination). The results showed that ND, HI HI, IB HI and AGD antibody titer in the experimental group were significantly higher than those in the control group at 7, 14, 21, 28, 35 and 42 days after inoculation (P <0.05) and increased 1.1 ~ 1.4, 1.0 ~ 1.3 and 0.7 ~ 1.4,0.9 ~ 1.6 respectively. The concentrations of IL-2, IFN-γ and IL-4 in the experimental group were significantly increased at 7, 14 and 21 days Higher than the control group and the blank group (P <0.05), the concentration of IL-10 in each group was not significantly different. The results show that TF can enhance the immune effect of NDV live vaccine, AIV inactivated vaccine, IBV inactivated vaccine and IBDV live vaccine.