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目的探讨SARS患者和康复者的血清学和病原学变化;筛选SARS康复者淋巴细胞中差异表达的基因。方法ELISA、冠状病毒全基因组芯片、抑制消减杂交(SSH)。结果80.4%的SARS康复者和2%的密切接触者SARSCoV的IgG抗体为阳性,康复后8个月内康复者抗体平均水平呈下降趋势;芯片能检测出血、便、痰和尿标本中的冠状病毒核酸,发现编码S1蛋白的核酸在大部分标本中是连续的;SSH获得了SARS康复者淋巴细胞中差异表达的77个cDNA克隆,其中4个为未知的新序列。结论大多数SARS康复者产生了冠状病毒特异的IgG抗体;密切接触者中可能存在隐性感染者;冠状病毒全基因组芯片能检测出各标本中的病毒核酸并建立相应的基因谱;SSH文库为筛选新型冠状病毒特异的抗体基因奠定了分子基础。
Objective To investigate the serological and etiological changes of patients with SARS and those who recover from SARS and to screen the differentially expressed genes in lymphocytes of SARS-ex-vivo patients. Methods ELISA, coronavirus whole genome chip, suppression subtractive hybridization (SSH). Results The IgG antibody of SARSCoV was positive in 80.4% of the SARS patients and 2% of the close contacts, and the average antibody level of the patients recovered within 8 months after rehabilitation showed a decreasing trend. The chip could detect corona in the samples of hemorrhage, stool, sputum and urine The viral nucleic acid was found to be contiguous in most specimens of the nucleic acid encoding the S1 protein. SSH generated 77 cDNA clones that were differentially expressed in the lymphocytes of SARS survivors, four of which were unknown new sequences. Conclusions Most SARS patients have coronavirus-specific IgG antibodies; there may be latent infection in close contacts; coronavirus genome-wide microarray can detect the viral nucleic acid in each sample and establish the corresponding gene spectrum; the SSH library is Screening of novel coronavirus-specific antibody genes laid the molecular basis.