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目的:探讨缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在家族性腺瘤性息肉病(FAP)患者结肠腺瘤、癌变腺瘤中的表达变化及相关性,分析其对家族性腺瘤性息肉病“腺瘤-腺癌”发生发展的作用。方法:应用免疫组化SP法分别检测10例家族性腺瘤性息肉病患者术后切除标本中正常上皮、≤0.5cm腺瘤、≥1.0cm腺瘤以及癌变腺瘤中HIF-1α和VEGF表达情况,并分析二者与临床病理特征之间关系。结果:在正常上皮黏膜、≤0.5cm腺瘤、≥1.0cm腺瘤以及癌变腺瘤变化过程中两者表达逐渐增强(P均大于0.05),其表达与腺瘤体积有关,癌变腺瘤与正常黏膜之间表达有显著差异(P<0.05)。≤0.5cm腺瘤与正常黏膜之间无显著差异,但癌变腺瘤与两者之和有显著差异(P<0.05)。两者表达水平呈现极显著正相关(P<0.01)。结论:HIF-1α、VEGF在家族性腺瘤性息肉病“腺瘤-腺癌”发展中起重要作用,HIF-1α、VEGF表达存在显著的正相关,表明在家族性腺瘤性息肉病“腺瘤-腺癌”进程中,VEGF的表达主要由HIF-1α水平调控。
Objective: To investigate the expression and correlation of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in patients with adenomatous adenoma and adenoma of familial adenomatous polyposis (FAP) The role of familial adenomatous polyposis “adenoma - adenocarcinoma” occurred in the development. Methods: The expressions of HIF-1α and VEGF in normal epithelium, ≤0.5cm adenoma, ≥1.0cm adenoma and cancerous adenoma were detected by immunohistochemical SP method in 10 cases of familial adenomatous polyposis patients , And analyze the relationship between the two and clinicopathological features. Results: The expressions of adenocarcinoma and normal adenocarcinoma were significantly increased in normal epithelial mucosa, ≤0.5cm adenoma, ≥1.0cm adenoma and adenocarcinoma (P> 0.05) Mucosal expression was significantly different (P <0.05). There was no significant difference between adenomas ≤0.5cm and normal mucosa, but there was significant difference between the two groups (P <0.05). There was a significant positive correlation between the two expression levels (P <0.01). Conclusions: HIF-1α and VEGF play an important role in the development of familial adenomatous polyposis “adenoma-adenocarcinoma”. There is a significant positive correlation between the expression of HIF-1α and VEGF, indicating that in familial adenomatous polyposis, In the process of “adenoma-adenocarcinoma”, the expression of VEGF is mainly regulated by the level of HIF-1α.