论文部分内容阅读
目的 :探讨阳离子脂质体介导的神经营养因子 3(NT3)基因转染对豚鼠庆大霉素性耳聋的保护作用。方法 :将携带外源性基因NT3的重组真核表达载体 pIRES2 EGFP NT3与阳离子脂质体复合物注入豚鼠耳蜗 ,术后给予庆大霉素肌肉注射 (12 0mg/kg) 14d ,分别于停药后 1d、2周进行听性脑干反应 (ABR)及畸变产物耳声发射 (DPOAE)测听并观察转染基因在耳蜗的表达和分布及耳蜗毛细胞受损情况。结果 :NT3组DP gram幅值降低较对照组明显减轻 ,ABR阈值升高亦无对照组显著 (P <0 .0 5 )。耳蜗基底膜FITC phalloidin染色见NT3组耳蜗毛细胞的缺失较对照组明显减轻。结论 :阳离子脂质体介导的NT3基因转染对豚鼠庆大霉素性耳聋具有一定程度的保护作用。
Objective: To investigate the protective effect of cationic liposome-mediated transfection of NT3 gene on gentamicin-induced deafness in guinea pigs. Methods: Recombinant eukaryotic expression vector pIRES2 EGFP NT3 carrying the exogenous gene NT3 was injected into the cochlea of guinea pig cochlea with intramuscular injection of gentamicin (12 0 mg / kg) for 14 days, Auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) were measured at 1 day and 2 weeks after operation. The expression and distribution of transfection gene in cochlea and the damage of cochlear hair cells were observed. Results: The amplitude of DP gram in NT3 group was significantly lower than that in control group, and the increase of ABR threshold was not significant in control group (P <0.05). Cochlear basement membrane FITC phalloidin staining NT3 group cochlear hair cell loss was significantly reduced compared with the control group. CONCLUSION: Cationic liposome-mediated NT3 gene transfection has a protective effect on gentamicin-induced deafness in guinea pigs.