全球大约10%~15%的育龄夫妇不育,男性因素约占50%。男性不育众多因素中遗传学因素占约10%~([1]),Y染色体微缺失是临床常见的遗传学病因。Y染色体长臂q11上存在控制精子生成的基因或基因家族,称为无精子因子(azoospermia facor,AZF)。AZF区域存在4个亚区(AZFa、AZFb、AZFc和AZFd),它们与精子生成相关,不同区域 About 10% to 15% of the world’s infertility couples are infertile, male factors account for about 50%. Many factors of male infertility, genetic factors accounted for about 10% ~ ([1]), Y chromosome microdeletions is a common clinical genetics. There is a gene or gene family that controls spermatogenesis in the long arm of chromosome Y, called azoospermia facor (AZF). There are 4 sub-regions (AZFa, AZFb, AZFc and AZFd) in AZF region, which are related to spermatogenesis. Different regions