论文部分内容阅读
目的观察十子代平方对T_2DM GK大鼠糖代谢的影响及对α-葡萄糖苷酶的抑制作用。方法 SPF级雄性13周龄GK大鼠,适应性喂养1周后给予高脂饲料连续喂养4周,以随机血糖或糖负荷2h血糖≥11.1mmol/L为成模标准,将成模GK大鼠随机分为模型组、阿卡波糖组(13.38mg/kg)、十子代平方低、中、高剂量组(分别含生药4、8、16g/kg),每组10只;另选等量Wistar大鼠为正常组,给予普通饲料喂养。给药前检测GK大鼠葡萄糖耐量(OGTT),给药前、给药4、8周分别检测空腹血糖(FBG),给药8周检测糖化血红蛋白(HbA1c)。另外,以蔗糖为底物,建立α-葡萄糖苷酶抑制剂体外筛选模型,阿卡波糖为阳性对照药,开展十子代平方抑制α-葡萄糖苷酶的实验研究,观察其对α-葡萄糖苷酶活性的影响。结果十子代平方能够降低GK大鼠空腹血糖和餐后2h血糖、降低GK大鼠FBG及HbA1c水平,当浓度为125mg/ml时α-葡萄糖苷酶抑制活性最强;十子代平方、阿卡波糖抑制α-葡萄糖苷酶作用的IC50值分别为:16.057、0.376mg/ml。结论十子代平方能够降低T_2DM GK大鼠空腹血糖和餐后2h血糖,改善GK大鼠FBG、HbA1c水平,促进体内糖代谢;十子代平方能够抑制α-葡萄糖苷酶活性,这可能是该方降血糖的主要作用机制之一。
Objective To observe the effect of the ten descendants and squares on the glycometabolism in T 2 DM GK rats and its inhibitory effect on α-glucosidase. Methods SPF male 13-week-old GK rats were fed with high fat diet one week after adaptive feeding for 4 weeks. Random GG rats were randomly divided into two groups: random blood glucose or blood sugar> 11.1mmol / L, Divided into model group, acarbose group (13.38mg / kg), ten generations of square low, middle and high dose group (containing crude drug 4,8,16g / kg), each group of 10; Wistar rats as normal group, fed with normal feed. The glucose tolerance (OGTT) of GK rats was measured before administration. Fasting blood glucose (FBG) was measured at 4 weeks and 8 weeks after administration, and HbA1c was measured at 8 weeks. In addition, sucrose as a substrate, the establishment of α-glucosidase inhibitor screening model in vitro, acarbose as a positive control drug to carry out the ten generations of square inhibition of α-glucosidase experimental study to observe its α-glucose Glycosidase activity. Results The results showed that ShiZaiJingFang can reduce fasting plasma glucose and 2h postprandial blood glucose in GK rats, and reduce FBG and HbA1c levels in GK rats. When the concentration was 125mg / ml, α-glucosidase inhibitory activity was the strongest; The IC50 values of kappo sugar inhibiting α-glucosidase were 16.057,0.376mg / ml respectively. Conclusions Shiziyi square can reduce fasting plasma glucose and 2h postprandial blood glucose, improve FBG and HbA1c levels and promote glucose metabolism in T 2 DM rats, which may inhibit the activity of α-glucosidase One of the main mechanism of hypoglycemic effect.