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目的:观察低氧(Hypoxia,Hyp)对大鼠视网膜神经节转化细胞(retinaganglioncell-5,RGC-5)氧化应激损伤的影响及牛磺酸(Taurine,Tau)的防护效应。方法:将RGC-5置于低氧条件(5%O2,5%CO2,90%N)2,加入不同浓度的牛磺酸(0.05mM、0.1mM、0.5mM、1mM)预处理后培养12h,24h和48h,使用MTT法检测细胞活力,并通过对NO、GSH、MDA等指标的检测,观察牛磺酸对RGC-5的保护效应。结果:低氧处理后RGC-5细胞活力明显降低(P<0.05),牛磺酸处理组细胞活力明显高于低氧组,其中0.1mM牛磺酸组作用最为显著(P<0.05);低氧组与常氧组比较,RGC-5的NO、GSH含量明显降低(P<0.05),而MDA含量显著升高(P<0.05);牛磺酸处理组与低氧组比较,RGC-5细胞GSH,NO的含量显著升高(P<0.05),而MDA的含量显著降低(P<0.05)。结论:牛磺酸能有效增强低氧损伤中RGC-5细胞的活力,其机制可能与牛磺酸可以提高其抗氧化能力有关。
Objective: To observe the effects of hypoxia on the oxidative stress injury of retina ganglioncell-5 (RGC-5) cells and the protective effect of Taurine (Tau) on rat retinal ganglion cells. METHODS: RGC-5 cells were cultured under hypoxic conditions (5% O2, 5% CO2, 90% N) 2 for 12h after pretreatment with different concentrations of taurine (0.05mM, 0.1mM, 0.5mM, , 24h and 48h respectively. MTT assay was used to detect cell viability. The protective effects of taurine on RGC-5 were observed by detecting NO, GSH and MDA. Results: The viability of RGC-5 cells was significantly decreased after hypoxia treatment (P <0.05), and the viability of taurine-treated cells was significantly higher than that of hypoxia group (0.1mM taurine) (P <0.05) Compared with normoxia group, the content of NO and GSH in RGC-5 group was significantly decreased (P <0.05) and MDA content was significantly increased (P <0.05). Compared with hypoxia group, RGC-5 The content of GSH and NO in cells increased significantly (P <0.05), while MDA content decreased significantly (P <0.05). CONCLUSION: Taurine can effectively enhance the vitality of RGC-5 cells in hypoxia. The mechanism may be related to the ability of taurine to enhance its antioxidant capacity.