论文部分内容阅读
目的建立简便、快速的筛选方法,获得具有腈水解酶活性的真菌,并用筛选获得的菌株对氰基吡啶类化合物进行转化。方法建立了基于腈水解反应中体系pH变化为指示的微量比色筛选方法,应用筛选获得的睛水解酶活性菌对2-氰基吡啶、3-氰基吡啶、4-氰基吡啶、蓖麻碱4种化合物进行转化。结果应用微量比色筛选法从23株真菌中筛选获得一株具有腈水解酶活性的真菌As-pergillus niger 3.795;该菌株对4-氰基吡啶表现出较高活性,其全细胞转化4-氰基吡啶生成4-吡啶甲酸(异烟酸)转化率可达84%,且没有检测到副产物。
OBJECTIVE To establish a simple and rapid screening method to obtain fungi with nitrilase activity and to convert cyanopyridines using the selected strains. Methods Based on the micro-colorimetric screening method based on the change of pH in the system of nitrile hydrolysis, the enzyme activity of 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine and castor Alkali 4 compounds were transformed. Results Aspergillus niger 3.795, a fungus with nitrilase activity, was screened from 23 fungi by micro-colorimetric screening. The strain showed high activity for 4-cyanopyridine, and the whole cell was transformed into 4-cyano Pyridine to 4-pyridine carboxylic acid (isonicotinic acid) conversion rate of up to 84%, and no by-products detected.