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研究肿瘤坏死因子（ＴＮＦ）影响单核细胞与体外培养的牛脑微血管内皮细胞（ＢＣＭＥＣ）粘附，迁移的动力学过程及药物的保护作用．利用培养在胶原层上的单层内皮细胞，通过对粘附于内皮细胞单层上的单核细胞及胶原层中的单核细胞计数，测定单核细胞粘附及迁移．ＴＮＦ（１００ｋＵ·Ｌ－１）可促进单核细胞与ＢＣＭＥＣ的粘附及迁移，其促粘附与迁移作用具明显的时效关系．ＴＮＦ促粘附达最大效应时间为２ｈ，较对照提高１２０．７％；ＴＮＦ促迁移作用１ｈ达到坪值，提高９１．９％．药物戊烯氧呋豆素（ｉｍ－ｐｅｒａｔｏｒｉｎ，ＩＭＰ），己酮可可碱（ｐｅｎｔｏｘｉｆｙｌｉｎｅ，ＰＴＸ）抑制由ＴＮＦ引起的单核细胞与ＢＣＭＥＣ的粘附及迁移，在浓度为１，１０及１００μｍｏｌ·Ｌ－１时，其粘附抑制率分别为７３．１％，８７．７％，９３．３％和７３．６％，８７．２％，８９．０％；迁移抑制率分别为１７．４％，４３．４％，６８．１％和３７．７％，４９．３％，６２．３％．ＩＭＰ和ＰＴＸ能抑制单核细胞与ＴＮＦ诱导的ＢＣＭＥＣ的粘附及迁移．
To study the effect of tumor necrosis factor (TNF) on the adhesion and migration of monocytes and cultured bovine microvascular endothelial cells (BCMECs) in vitro and the protective effect of drugs. Monocyte adhesion and migration were measured by counting monocytes adherent to monolayers of endothelial cells and monocytes in the collagen layer using monolayers of endothelial cells cultured on collagen layers. TNF (100kU · L-1) can promote the adhesion and migration of monocytes and BCMEC, and its adhesion-promoting and migration-inducing effect have obvious time-effect relationship. The maximum effect time of TNF up-regulation was 2h, which was 120.7% higher than that of the control. The secretion of TNF increased to 91.9% after 1h incubation. The drugs im-peratorin (IMP) and pentoxifylline (PTX) inhibited the adhesion and migration of monocytes and BCMECs induced by TNF at concentrations of 1, 10 and 100 μmol·L -1, the adhesion inhibition rates were 73.1%, 87.7%, 93.3% and 73.6%, 87.2% and 89.0% respectively. The migration inhibition rates were 17.4% , 43.4%, 68.1% and 37.7%, 49.3% and 62.3% respectively. IMP and PTX inhibited the adhesion and migration of monocytes to TNF-induced BCMEC.