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利用自聚合法合成聚乙烯吡咯烷酮包金(Au@PVP)核壳纳米粒子,利用壳层PVP分子分散纳米粒子的特性,使其形成均一、排列致密的单层结构。氯霉素分子通过氢键吸附在Au@PVP核壳粒子SERS基底上。优化激发光波长,利用金纳米内核的等离子体共振效应实现了对氯霉素分子的高灵敏度表面增强拉曼检测,检测限可以达到10~(-10) mol/L。将建立的方法用于蜂蜜样品中氯霉素的检测,氯霉素含量在(4.4×10~(-9)~4.9×10~(-5))mol/L,样品回收率在88%~101.12%,相对标准偏差在3.51%~12.44%。结果表明该方法快速准确、操作简单,可以用于蜂蜜中氯霉素的快速检测。
Polyvinylpyrrolidone (Au @ PVP) core-shell nanoparticles were synthesized by self-polymerization method. The properties of the nanoparticles dispersed in the shell PVP molecules were used to form uniform and dense monolayer structure. Chloramphenicol molecules are hydrogen-bonded to the SERS substrate of Au @ PVP core-shell particles. The wavelength of excitation light is optimized, and the high sensitivity surface-enhanced Raman detection of chloramphenicol molecules is realized by using the plasmon resonance effect of the gold nanocrystal core. The detection limit can reach 10 -10 mol / L. The established method was applied to the detection of chloramphenicol in honey samples. The content of chloramphenicol was between 4.4 × 10 -9 ~ 4.9 × 10 -5 mol / L and the recovery rate was between 88% 101.12% with relative standard deviation of 3.51% ~ 12.44%. The results show that the method is rapid and accurate, easy to operate and can be used for the rapid detection of chloramphenicol in honey.