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目的:探讨益气补肾活血方对大鼠退变腰椎间盘中OP-1及IL-1的影响。方法:将60只大鼠随机分为对照组、实验组、假手术组,每组20只;采用腰椎间盘纤维环穿刺法建立腰椎间盘退变模型,术后第1天,实验组予益气补肾活血中药浓缩液灌胃,术后第4、8周,每组各取5只大鼠,HE染色观察椎间盘组织形态学改变,ELISA法观察椎间盘中OP-1、IL-1含量。结果:HE染色结果可见实验组大鼠椎间盘退变程度低于对照组;实验组及对照组大鼠造模后4、8周椎间盘中OP-1含量均低于假手术组(P<0.05),IL-1含量均高于假手术组(P<0.05);实验组大鼠腰椎间盘中OP-1含量高于对照组(P<0.05),IL-1含量低于对照组(P<0.05)。结论:益气补肾活血方可调节大鼠退变腰椎间盘中OP-1及IL-1的含量,减缓椎间盘退变。
Objective: To investigate the effects of “Yiqi Bushen Huoxue Decoction” on the expression of OP-1 and IL-1 in degenerated lumbar intervertebral disc in rats. Methods: Sixty rats were randomly divided into control group, experimental group and sham operation group, with 20 rats in each group. The lumbar intervertebral disc degeneration model was established by lumbar intervertebral disc annulus puncture. On the first day after operation, Bushen Huoxue Chinese medicine concentrates were given orally. At the 4th and 8th week after the operation, 5 rats in each group were taken. HE staining was used to observe the morphological changes of the intervertebral discs. The contents of OP-1 and IL-1 in the intervertebral discs were observed by ELISA. Results: HE staining showed that the degree of disc degeneration in the experimental group was lower than that in the control group. The contents of OP-1 in the intervertebral disc of the experimental group and the control group were lower than those of the sham operation group 4 and 8 weeks after modeling (P <0.05) (P <0.05). The content of OP-1 in the lumbar intervertebral disc in the experimental group was higher than that in the control group (P <0.05), while the content of IL-1 in the experimental group was lower than that in the control group ). Conclusion: Yiqi Bushen Huoxue Fang can regulate the content of OP-1 and IL-1 in degenerative lumbar intervertebral disc and slow down the degeneration of intervertebral disc.