论文部分内容阅读
将去除和保留内皮细胞的门静脉 ,分别置于 4℃的 UW( U niversity of Wisconsin)液和 Krebs液中保存 ,在 36℃ Krebs液和去甲肾上腺素作用条件下观察不同保存时间对门静脉收缩力的影响 .结果表明 :去除内皮细胞的门静脉 ,无论是在 Krebs液中还是在 UW液中保存 ,其收缩力均随保存时间的延长而下降 ,72 h后分别为 30 %和 6 5 % ;而保留内皮细胞的门静脉 ,其收缩力均随保存时间的延长而增大 ,Krebs液中保存优于 UW液 ,72 h后分别为 2 1 3%和 1 1 8% .无论有无内皮细胞 ,门静脉在 UW液和 Krebs液中保存 ,其收缩力以 UW液中保存的最接近刚离体时的水平 .
The portal vein with endothelial cells removed and preserved was stored in UU (University of Wisconsin) solution and Krebs solution at 4 ° C. The effects of different preservation time on the contractility of portal vein were observed under Krebs fluid and noradrenaline treatment at 36 ℃. .The results showed that the contraction of endothelial cells in the Krebs solution or UW solution decreased with the prolongation of storage time and reached 30% and 65% after 72 h, respectively The preservation of endothelial cells in the portal vein, the contractile force increased with the preservation of time, preservation better than UW solution in Krebs solution after 72 h were 21.3% and 118% with or without endothelial cells, portal vein In UW solution and Krebs solution, the contractile force is stored in the UW fluid, the closest to just the body when the level.