新显色剂间氯偶氮安替比林在pH 5.85条件下与铜生成蓝色络合物,用连续浓度变化法和摩尔比法测定,其组成为1:1,该反应对铜之测定有较高灵敏度。吸光度可在45min内保持不变。本文研究了间氯偶氮安替比林与铜反应之较佳条件,稳定常数为2.1×10~6,在波长640nm处有最大吸收,摩尔吸光率为2.5×10~4,25ml溶液中含铜0～25μg遵守比耳定律。检出下限0.5μg。一仪器与试剂 721s型分光光度计间氯偶氮安替比林溶液:0.04%,称取0.4g固体试剂加水溶解稀至1L(过滤后使用)。六偏磷酸钠溶液:5% 缓冲溶液:0.1M乙酸钠溶液320ml与0.1M乙酸 The new reagent, meta-chloroantibiotin, is blue-complexed with copper at pH 5.85 and has a composition of 1: 1 using a continuous concentration change method and a molar ratio method. The determination of copper Have a higher sensitivity. Absorbance can remain unchanged within 45min. In this paper, the best conditions for the reaction between m-chloroantipyrine and copper were studied. The stability constant was 2.1 × 10 ~ 6, the maximum absorption was at 640nm, the molar absorptivity was 2.5 × 10 ~ 4, Copper 0 ~ 25μg obey Beer’s law. The detection limit of 0.5μg. An instrument and reagent 721s spectrophotometer chloroantipyrine antipyrine solution: 0.04%, weighed 0.4g solid reagent diluted with water to 1L (used after filtration). Sodium hexametaphosphate solution: 5% buffered solution: 0.1 M sodium acetate solution 320 ml and 0.1 M acetic acid