论文部分内容阅读
我们用ＥＬＩＳＡ对２２例接受抗ＣＥＡ单抗Ｃ（５０）放射免疫显像的卵巢癌病人进行了血清人抗鼠抗体（ＨＡＭＡ）产生的动态检测。４５．５％（１０／２２）的患者产生ＨＡＭＡ，人抗鼠抗体在给药后２～３周即可出现，４～５周达高峰，并在体内持续至少９个月。所产生的ＨＡＭＡ在双向琼脂扩散中仅与抗人ＩｇＧ有沉淀线，且与标准ＩｇＧ的沉淀线相连，与抗人Ｉｇ轻链ｋ、λ单抗、葡萄球菌Ａ蛋白、羊抗人ＩｇＧ均起反应，证明该抗体属于ＩｇＧ。
We performed dynamic detection of serum human anti-mouse antibody (HAMA) production in 22 patients with ovarian cancer who received anti-CEA monoclonal antibody C (50) radioimmunoimaging using ELISA. 45.5% (10/22) of patients developed HAMA. Human anti-mouse antibodies appeared 2 to 3 weeks after dosing, peaked at 4 to 5 weeks, and persisted in the body for at least 9 months. The resulting HAMA was only precipitated with anti-human IgG in the two-dimensional agar diffusion, and was linked to the precipitation line of standard IgG, and both of the anti-human Ig light chain k, lambda monoclonal antibody, staphylococcal protein A and goat anti-human IgG The reaction proved that the antibody belongs to IgG.