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目的 射频消融治疗肝脏肿瘤的机制目前尚不明了 ,我们通过本实验观察射频治疗后兔肿瘤模型及临床肝癌病例的分子生物学改变。方法 实验兔 3 2只制成VX2 细胞肝癌模型 ,分为射频组 ( 2 8只 )即术后 0、2、6、12、2 4、48、168h和对照组 ( 4只 ) ,通过HE染色、TUNEL法及二苯胺法观察肿瘤组织及治疗旁中心区组织的凋亡指数改变。结果 射频组肿瘤经射频治疗后发生凝固性坏死及细胞凋亡 ,凝固性坏死边缘区肿瘤组织凋亡指数明显高于对照组 ,其指数术后 0h即升高 ,术后 12h均值达高峰 (P <0 .0 1) ,后逐渐下降 ,但术后 168h仍高于术前组。结论 射频治疗通过诱导高温凝固性坏死区周边肿瘤细胞凋亡 ,进一步扩大了射频治疗的治疗范围 ,诱导细胞凋亡是射频治疗治疗恶性肿瘤的一个重要机制
Purpose The mechanism of radiofrequency ablation for the treatment of liver tumors is not yet clear. We observed the molecular biology changes of rabbit tumor models and clinical liver cancer patients after radiofrequency ablation by this experiment. Methods 32 rabbits were randomly divided into radiofrequency group (28), which were 0, 2, 6, 12, 2, 48, 168 h after operation and control group (4) TUNEL method and diphenylamine method were used to observe the changes of apoptosis index in the tumor tissue and the para-center tissue. Results The RFA group had coagulation necrosis and apoptosis after radiofrequency ablation. The apoptotic index in the edge of coagulation necrosis group was significantly higher than that in the control group. The index rose at 0h after surgery and peaked at 12h <0. 01), and then gradually decreased, but 168h after surgery still higher than the preoperative group. Conclusion Radiofrequency treatment can further increase the therapeutic range of radiofrequency ablation by inducing apoptosis of peripheral tumor cells in the area of high temperature coagulation necrosis and inducing apoptosis is an important mechanism of radiofrequency therapy in the treatment of malignant tumors