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目的:探讨刺五加皂苷对血管性痴呆大鼠脑组织微血管密度(MVD)及血管内皮细胞生长因子(VEGF)蛋白及VEGFmRNA表达的影响。方法:选用40只SD雄性健康大鼠,随机分为4组,采用颈内动脉注射微小栓子方法制作多发梗死性痴呆大鼠动物模型,进行Morris水迷宫行为学测试和脑组织形态学筛选评估模型,将造模成功大鼠予以刺五加皂苷干预,免疫组化检测大鼠脑切片CD34阳性MVD和VEGF的表达;实时荧光定量PCR法检测VEGF mRNA的动态变化。结果:免疫组化结果显示模型组和刺五加皂苷组CD34、VEGF表达均较假手术组、对照组增多(P<0.01);刺五加皂苷组与模型组相比较CD34表达增多(P<0.01),VEGF表达增多(P<0.05);实时荧光定量PCR结果显示模型组与刺五加皂苷组的VEGF mRNA表达均有显著差异,且刺五加皂苷组比模型组表达明显增多(P<0.05)。结论:刺五加皂苷可促进毛细血管新生,改善血管性痴呆大鼠的记忆能力,机制可能是刺激VEGF表达。
Objective: To investigate the effects of acanthopanax senticosus on the microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor (VEGF) mRNA in the brain of vascular dementia rats. Methods: Forty male Sprague-Dawley rats were randomly divided into four groups. The animal model of multiple infarct dementia was made by injecting tiny emboli into the internal carotid artery. Morris water maze test and brain morphological evaluation Model. The successful model rats were given Acanthopanax senticosus intervention. The expression of CD34 positive MVD and VEGF in brain sections of rats were detected by immunohistochemistry. The dynamic changes of VEGF mRNA were detected by real-time fluorescence quantitative PCR. Results: The results of immunohistochemistry showed that the expressions of CD34 and VEGF in model group and acanthopanax senticosus group were higher than those in sham-operation group and control group (P <0.01); CD34 expression was increased in acanthopanax senticosus group compared with model group (P < (P <0.05). The results of real-time fluorescence quantitative PCR showed that the expression of VEGF mRNA in model group and Acanthopanax senticosus group were significantly different, and the acanthopanax senticosus group was significantly higher than the model group (P < 0.05). Conclusion: Acanthopanax senticosus can promote angiogenesis and improve the memory ability of vascular dementia rats. The mechanism may be to stimulate VEGF expression.