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AIM:To examine whether 2’-5’oligoadenylate synthetase (OAS) gene promoter can be specifically activated by hepatitis C virus (HCV)-core protein.METHODS: Human embryo hepatic cell line L02 wa transfected with pcDNA3.1-core plasmid and selected by G418. Expression of HCV-core was detected by reverse transcription polymerase chain reaction and Western blotting. The OAS promoter sequence wa amplified from the genomic DNA and inserted into pGL3-basic vector. The resultant pGL3-OAS-Luc plasmid was transiently transfected into L02/core cell and luciferase activity was assayed. RESULTS: L02/core cell line stably expressing HCV core protein was established. The pGL3-OAS-Luc construct exhibited significant transcriptional activity in the L02/core cells but not in the L02 cells.CONCLUSION: HCV-core protein activates the OAS gene promoter specifically and effectively. Utilization of OAS gene promoter would be an ideal strategy for developing HCV-specific gene therapy.
AIM: To examine whether 2’-5’oligoadenylate synthetase (OAS) gene promoter can be specifically activated by hepatitis C virus (HCV) -core protein. METHODS: Human embryo hepatic cell line L02 wa transfected with pcDNA3.1-core plasmid and selected by G418. Expression of HCV-core was detected by reverse transcription polymerase chain reaction and Western blotting. The OAS promoter sequence wa amplified from the genomic DNA and inserted into pGL3-basic vector. The resultant pGL3-OAS-Luc plasmid was transiently transfected L02 / core cell and luciferase activity was assayed. RESULTS: L02 / core cell line stably expressing HCV core protein was established. The pGL3-OAS-Luc construct showed significant transcriptional activity in the L02 / core cells but not in the L02 cells. CONCLUSION: HCV-core protein activates the OAS gene promoter specifically and effectively. Utilization of OAS gene promoter would be an ideal strategy for HCV-specific gene therapy.