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孕前检测育龄妇女巨细胞病毒感染的情况,对优生优育有重要意义.自1996年6月开始,定陶县计划生育服务站应用ELISA法检测育龄妇女血清中的HCMV-IgM,对全县安排计划内生育的妇女孕前感染情况进行了调查,现将筛查结果报道如下.1 材料与方法1.1 调查对象 1998年对计划内生育的育龄妇女进行检测,包括计划内一胎(简称一胎)者,年龄在23～25周岁,计划内二胎(简称二胎)者,年龄在30～32周岁和有过自然流产、早产、死胎及胎儿畸型等异常妊娠史者,年龄在25～30周岁.1.2 方法 育龄妇女在办理生育证前抽取肘静脉血2ml,低温2～8℃保存待测定.应用山东省潍坊康华生物技术有限公司生产的HCMV-IgM抗体酶联免疫检测试剂盒,定性检测血清中HCMV-IgM.操作步骤、结果判断、注意事项,均严格按照说明书进行.2 结果共检测育龄妇女3000人,HCMV平均阳性率为17.5%.其中,一胎育龄妇女1400名,HCMV-IgM阳性者196名,阳性率为14%;二胎育龄妇女1500名,
Pre-pregnancy detection of women of childbearing age cytomegalovirus infection, prenatal and postnatal care of great significance.From June 1996 onwards, Dingtao County Family Planning Service Station ELISA detection of women of childbearing age serum HCMV-IgM, the county to arrange plans Infertility women pre-pregnancy infection were investigated, the results of the screening are reported as follows.1 Materials and methods 1.1 survey In 1998 the planned childbearing women of childbearing test, including the planned one-child (referred to as one-child) Age 23 to 25 years of age, planned second child (referred to as second child), aged 30 to 32 years old and had a spontaneous abortion, premature birth, stillbirth and fetal malformations and other abnormal pregnancy history, aged 25 to 30. 1.2 Methods Women of childbearing age to extract elbow venous blood 2ml before treatment of fertility, low temperature 2 ~ 8 ℃ stored to be determined. Application HCT-HCMV produced by Shandong Weifang Kanghua Biotechnology Co., Ltd. enzyme-linked immunosorbent assay kit, qualitative detection of serum HCMV-IgM.Methods of operation, results judgment and precautions were all carried out in strict accordance with the instructions.2 Results A total of 3000 women of childbearing age were tested, the average positive rate of HCMV was 17.5%, among them, 1400 were of childbearing age, HCMV 196 V-IgM positive, the positive rate was 14%; 1,500 women of childbearing age,