香荚兰的幼茎在MS＋BA1μg／mL＋NAA5μg／mL培养基上培养40d，其表面形成白色、块状的愈伤组织。愈伤组织在MS＋2，4－D1μg／mL＋BA1μg／mL＋NAA4μg／mL＋2．5％sucrose的半固体培养基上快速增殖培养，培养4周后培养物的重量增加7倍左右；在相同培养基的悬浮培养中，培养4周后培养物的重量增加6～8倍。从不同的培养物中分离出香兰素并测定其含量。 The stems of Vanilla japonica were cultured on MS + BA1μg / mL + NAA5μg / mL medium for 40 days, forming white, massive callus on the surface. The calluses were rapidly proliferated on semi-solid medium with MS + 2,4-Dlg / mL + BA1 g / mL + NAA4 g / mL + 2.5% sucrose and the weight of the culture increased about 7-fold after 4 weeks of culture; After 4 weeks of culture, the weight of the culture increased 6 to 8 times. Vanillin was isolated from different cultures and assayed for content.