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目的探讨越橘提取物抑制HeLa细胞增殖和诱导凋亡作用及其可能机制。方法用浓度为0、0.025、0.25、2.5、25μg/mL的越橘提取物处理HeLa细胞24h后,采用四甲基偶氮唑盐(MTT)方法检测越橘提取物对HeLa细胞增殖的抑制作用,采用Hoechst33342/PI染色和DNAladder方法观察越橘提取物对HeLa细胞凋亡的诱导作用,并采用Western blotting法检测caspase-3和caspase-8蛋白表达。结果0.025、0.25、2.5和25μg/L的越橘提取物对HeLa细胞增殖的抑制率分别为22.81%、36.75%、42.62%和45.22%;0.25~25μg/L的越橘提取物处理组细胞呈现明显的凋亡改变;Western blotting结果显示越橘提取物处理组细胞中caspase-8和caspase-3酶原蛋白激活,出现裂解片断。结论越橘提取物可通过诱导HeLa细胞凋亡而抑制其增殖。
Objective To investigate the effect of bilberry extract on the proliferation and apoptosis induction of HeLa cells and its possible mechanism. Methods Hela cells were treated with bilberry extract at concentrations of 0, 0.025, 0.25, 2.5, and 25 μg/mL for 24 h. MTT assay was used to detect the inhibitory effect of bilberry extract on the proliferation of HeLa cells. The Hoechst33342/PI staining and DNA ladder methods were used to observe the effect of bilberry extract on the apoptosis of HeLa cells. The expression of caspase-3 and caspase-8 protein was detected by Western blotting. Results The inhibitory rates of 0.025, 0.25, 2.5, and 25 μg/L bilberry extracts on HeLa cells were 22.81%, 36.75%, 42.62%, and 45.22%, respectively. The cells in the biloba extract treatment group at 0.25-25 μg/L were present. Obvious changes in apoptosis; Western blotting results showed that the caspase-8 and caspase-3 zymogen proteins in the cells of the bilberry extract treatment group were activated and showed cleavage fragments. Conclusion Bilberry extract can inhibit the proliferation of HeLa cells by inducing apoptosis.