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羊肚菌[Morchella esculenta(L.)Pers.)]多糖具有降血脂及抗肿瘤功能。从羊肚菌培养液中得到分子量10000~100000万的多糖MEP-SP,采用DEAE-纤维素离子交换柱及Sepharose CL-6B柱,以水为洗脱液,分离得水溶性多糖MEP-SP1。实验结果表明,MEP-SP1是一种由木糖、葡萄糖、阿拉伯糖和半乳糖(摩尔比29:24:61:39)残基为重复单元组成的杂多糖,分子量约为11 500。从红外光谱(IR)及核磁共振氢谱(~1HNMR)看出主链包括β-吡喃糖苷键。
The Morchella esculenta (L.) Pers.) polysaccharide has hypolipidemic and anti-tumor properties. The polysaccharide MEP-SP with a molecular weight of 10,000 to 10 million was obtained from Morchella esculentum. The water-soluble polysaccharide MEP-SP1 was isolated using DEAE-cellulose ion exchange column and Sepharose CL-6B column with water as eluent. The experimental results show that MEP-SP1 is a heteropolysaccharide consisting of xylose, glucose, arabinose and galactose (molar ratio 29:24:61:39) residues as repeat units, with a molecular weight of approximately 11,500. From the infrared spectroscopy (IR) and nuclear magnetic resonance spectrum (~1H NMR), it was found that the main chain includes the β-pyranoside bond.