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目的:探讨Nogo-B基因在人脑胶质瘤组织中的表达及其与胶质瘤病理分级的相关性。方法:收集上海长征医院神经外科2005-2008年手术获得的经病理证实的人脑胶质瘤组织标本36份,另取其他手术切除的正常脑组织标本10份。以免疫组化技术、Real-time PCR和Western blotting等方法检测这些标本中Nogo-B在基因和蛋白质层面的表达水平,结合病理资料分析Nogo-B的表达和病理分级的相关性。结果:Real-time PCR检测发现,恶性程度高的胶质瘤组织中Nogo-B基因表达明显下调.Ⅲ级的胶质瘤组织中Nogo-B基因表达水平与正常脑组织相比下降了90%左右(P<0.01),Nogo-B基因表达水平与肿瘤的恶性度成相关。Western blotting检测显示,正常脑组织中Nogo-B蛋白表达相对恒定;在高恶性级别的胶质瘤中Nogo-B的蛋白表达显著下调,其下降程度与肿瘤的恶性程度呈负相关。免疫组织化学检测显示,Nogo-B主要在人正常脑组织中弥漫性阳性表达(100%),在人胶质瘤组织中表达显著降低;36例胶质瘤组织中Nogo-B染色阳性程度随着肿瘤恶性程度增加而显著降低(在Ⅰ-Ⅱ、Ⅲ、Ⅳ级中阳性表达分别为88.2%,42.9%,25.0%),各组间比较差异有统计学意义(P<0.01)。结论:在人脑胶质瘤中Nogo-B在mRNA和蛋白质水平上表达均出现下调,其下调程度与病理分级呈负相关。
Objective: To investigate the expression of Nogo-B gene in human glioma tissue and its correlation with pathological grade of glioma. Methods: Totally 36 specimens of human glioma confirmed by pathology were collected from Department of Neurosurgery, Shanghai Changzheng Hospital from 2005 to 2008, and 10 specimens from other normal brain tissues were collected. The expression of Nogo-B at the gene and protein level in these specimens was detected by immunohistochemistry, Real-time PCR and Western blotting. The correlation between Nogo-B expression and pathological grade was analyzed by pathological data. Results: Real-time PCR showed that the expression of Nogo-B gene was significantly down-regulated in glioma tissues with high degree of malignancy.The level of Nogo-B gene expression in grade III glioma tissues was decreased by 90% compared with normal brain tissue (P <0.01). The expression level of Nogo-B gene correlated with the malignancy of the tumor. Western blotting showed that the expression of Nogo-B protein in normal brain tissue was relatively constant. The protein expression of Nogo-B was significantly down-regulated in gliomas with high malignant grade, and the decrease was negatively correlated with the malignant degree of tumor. Immunohistochemistry showed that Nogo-B was mainly diffusely expressed in human normal brain tissue (100%), and was significantly decreased in human glioma tissues. The positive expression of Nogo-B in 36 glioma tissues was The malignant degree of the tumor increased significantly (P <0.01). The positive expression rates were 88.2%, 42.9% and 25.0% in grade Ⅰ-Ⅱ, Ⅲ and Ⅳ respectively. There was significant difference between the two groups (P <0.01). CONCLUSION: The expression of Nogo-B in human glioma is down-regulated both at mRNA and protein level, and its down-regulation is negatively correlated with pathological grade.