论文部分内容阅读
目的对西宁市生鲜乳当中金黄色葡萄球菌及其肠毒素污染情况进行调查,动态了解全市乳类及其制品的安全性,为今后进一步开展食品安全风险评估提供科学依据。方法采集西宁市辖区内的养殖场乳头奶202份、原料奶200份,以及乳制品生产企业成品奶200份,参照GB 4789.10-2010的方法对金黄色葡萄球菌进行定量及定性检验,同时对金黄色葡萄球肠毒素进行定性检验。结果乳头奶当中金黄色葡萄球菌检出率为28.71%,原料奶检出率为38.50%,成品乳未检出,原料奶检出率高于乳头奶及成品乳,差异有统计学意义(χ2=92.114,P<0.001)。在检出为阳性的58份乳头奶中,金黄色葡萄球菌污染范围10~99 CFU/ml的样品占48.28%,污染范围100~999 CFU/ml的样品占34.48%,污染范围1 000~9 999 CFU/ml的样品占17.24%。77份检出阳性的原料奶中金黄色葡萄球菌污染范围10~99 CFU/ml的样品占19.48%,100~999 CFU/ml的样品占49.35%,污染范围1 000~9 999 CFU/ml的样品占20.78%,超过10 000 CFU/ml的样品占10.39%,原料奶的污染程度高于乳头奶,差异有统计学意义(χ2=16.555,P<0.01)。乳头奶当中金黄色葡萄球菌肠毒素检出率为2.97%,原料奶检出率为1.00%,成品乳中未检出肠毒素,原料奶检出率高于乳头奶及成品乳,差异有统计学意义(χ2=7.009,P<0.05)。结论西宁市生鲜乳中金黄色葡萄球菌及其肠毒素污染情况不容乐观,尤其原料奶污染较为严重,今后应进一步加强奶源管理,避免由于金黄色葡萄球菌及肠毒素污染引起的食物中毒。
Objective To investigate the contamination of Staphylococcus aureus and its enterotoxin in the fresh milk in Xining City and get a dynamic understanding of the safety of milk and its products in the city so as to provide a scientific basis for further conducting the food safety risk assessment in the future. Methods Totally 202 dairy nipples, 200 raw milk and 200 finished dairy products were collected from the farms in Xining. According to the method of GB 4789.10-2010, the quantitative and qualitative tests of Staphylococcus aureus were carried out. Yellow grape enterotoxin qualitative test. Results The detection rate of Staphylococcus aureus in the nipple milk was 28.71%, the detection rate of raw milk was 38.50%, the finished milk was not detected, the detection rate of raw milk was higher than nipple milk and finished milk, the difference was statistically significant (χ2 = 92.114, P <0.001). Among the 58 nipples detected positive, the samples contaminated with Staphylococcus aureus ranged from 10 to 99 CFU / ml accounted for 48.28%, the samples with contaminated range from 100 to 999 CFU / ml accounted for 34.48% and the contamination ranged from 1 000 to 9 A sample of 999 CFU / ml accounted for 17.24%. 77 samples with positive staphylococcus aureus contamination ranged from 10 to 99 CFU / ml, 19.48%, 49.35% from 100 to 999 CFU / ml and from 1 000 to 9 999 CFU / ml The samples accounted for 20.78%. Samples containing more than 10 000 CFU / ml accounted for 10.39%. The contamination of raw milk was higher than that of nipple milk (χ2 = 16.555, P <0.01). Among the nipple milk, the detection rate of staphylococcal enterotoxin was 2.97%, the detection rate of raw milk was 1.00%, the enterotoxin was not detected in the finished milk, and the detection rate of raw milk was higher than that of nipple milk and finished milk Significance (χ2 = 7.009, P <0.05). Conclusions The contamination of Staphylococcus aureus and enterotoxin in raw milk of Xining City is not optimistic, especially the contamination of raw milk is more serious. In the future, the management of milk source should be further strengthened to avoid food poisoning caused by Staphylococcus aureus and enterotoxin contamination.