论文部分内容阅读
黄孢原毛平革菌（Ｐｈａｎｅｒｏｃｈａｅｔｅｃｈｒｙｓｏｓｐｏｒｉｕｍ）能产生降解木质素的胞外木质素过氧化物酶（ＬＩＰ） 和锰过氧化物酶（ ＭｎＰ）同工酶。为研究ＬＩＰ基因的转录调控机理， 对ＬＩＰ基因（ ＧＬＧ３ 和ＧＬＧ６） 的５′端上游序列进行亚克隆， 获得６ 个亚克隆ＤＮＡ 片段， 然后应用凝胶迁移率变动分析技术筛选能与菌体蛋白质专一性结合的ＤＮＡ片段。结果表明： ＬＩＰ基因ＧＬＧ６ 的５′端上游有一个约６７０ ｂｐ 的ＤＮＡ 片段能与总蛋白质组分专一性结合， 其核苷酸序列分析表明该片段可能含有蛋白质结合的序列特征。研究结果初步显示， 黄孢原毛平革菌可能存在有与ＬＩＰ基因上游某些顺式调控元件相互作用的蛋白质， 调控着ＬＩＰ基因的转录表达。
Phanerochaetechrysosporium produces extracellular lignin peroxidase (LIP) and manganese peroxidase (MnP) isoenzymes that degrade lignin. In order to study the mechanism of transcriptional regulation of LIP gene, we subcloned the 5 ’end sequences of LIP genes (GLG3 and GLG6) to obtain 6 subcloned DNA fragments. Then, Specific binding of DNA fragments. The results showed that a DNA fragment of about 670 bp upstream of the 5 ’end of LIP gene GLG6 specifically bound to the total protein component, and its nucleotide sequence analysis indicated that the fragment may contain sequence features of protein binding. The results of the preliminary study showed that there may be some proteins interacting with some cis-regulatory elements upstream of LIP gene to regulate the transcriptional expression of LIP gene.