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目的:探讨肝癌组织中微血管密度(microvascular density,MVD)、微血管面积(microvascular area,MVA)以及Piezo1的表达水平预测肝癌微血管侵犯(microvascular invasion,MVI)的临床价值。方法:应用免疫组织化学方法检测38例病理证实为肝癌患者的肝癌组织的CD34以及Piezo1的表达情况,计算基于CD34染色的MVD和MVA,分析MVI与MVD,MVA以及Piezo1因子的表达水平的相关性。结果:38例肝癌中,13例有微血管侵犯,定义MVI(+)组,25例无微血管侵犯,定义MVI(.)组。MVI(+)组的MVA及MVD均高于MVI(.)组,两组间差异有统计学意义(P=0.007,P=0.011)。MVD和MVA联合预测MVI的敏感性和特异性为100%和64%,较单一指标效能高。Piezo1在肝癌MVI(+)组阳性率高于MVI(.)组,两组间差异有统计学意义(P=0.032)。结论:MVD,MVA以及Piezo1的表达水平均与肝癌MVI具有一定的相关性,可以作为辅助诊断微血管侵犯的指标,Piezo1可以作为潜在的限制MVI的治疗靶点。
Objective: To investigate the clinical value of microvascular density (MVD), microvascular area (MVA) and the expression of Piezo1 in predicting liver cancer microvascular invasion (MVI). Methods: Immunohistochemistry was used to detect the expression of CD34 and Piezo1 in 38 HCC patients with hepatocellular carcinoma. The MVD and MVA based on CD34 staining were calculated. The correlation between MVI, MVD, MVA and Piezo1 expression was analyzed . RESULTS: Thirteen of 38 HCCs had microvascular invasion, defined as MVI (+) and 25 without microvascular invasion, defining the MVI (.) Group. MVA and MVD in MVI (+) group were significantly higher than those in MVI (P <0.05), the difference was statistically significant (P = 0.007, P = 0.011). MVD and MVA combined prediction of MVI sensitivity and specificity of 100% and 64%, higher than a single indicator of high performance. The positive rate of Piezo1 in MVI (+) group was higher than MVI group (P <0.05), the difference was statistically significant (P = 0.032). CONCLUSION: The expression of MVD, MVA and Piezo1 are both correlated with MVI of liver cancer and may be used as an index to assist in the diagnosis of microvascular invasion. Piezo1 may be used as a potential therapeutic target for the treatment of MVI.